Difference between revisions of "Phosphofructokinase type 1"
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* The Vm value is reported as <math>56 \pm 23</math> (nmol/min/mg protein). HeLa cells were harvested at a concentration of 65 mg protein/ml <ref name="moreno_2012"></ref>. Converting Vm to mM/min becomes <math> 0.00364 \pm 0.001495 </math> again in the presence of 140 <math>\text{mM K}^{+}</math>. | * The Vm value is reported as <math>56 \pm 23</math> (nmol/min/mg protein). HeLa cells were harvested at a concentration of 65 mg protein/ml <ref name="moreno_2012"></ref>. Converting Vm to mM/min becomes <math> 0.00364 \pm 0.001495 </math> again in the presence of 140 <math>\text{mM K}^{+}</math>. | ||
− | * Four Keq values have been reported in the SilicoTrypWiki (Wikipedia for insilico modelling of Trypanosome) for Phosphofructokinase: 308.4, 254, 1035, 800. Taking the mean and std. dev. of this value is <math> 599.35 \pm 329.38</math> <ref name="SilicoTryp">[[http://silicotryp.ibls.gla.ac.uk/wiki/ | + | * Four Keq values have been reported in the SilicoTrypWiki (Wikipedia for insilico modelling of Trypanosome) for Phosphofructokinase: 308.4, 254, 1035, 800. Taking the mean and std. dev. of this value is <math> 599.35 \pm 329.38</math> <ref name="SilicoTryp">[[http://silicotryp.ibls.gla.ac.uk/wiki/Phosphofructokinase|Silicotryp]]</ref> |
Revision as of 12:03, 24 April 2014
The enzyme Phosphofructokinase Type-1 uses another ATP molecule to transfer a phosphate group to Fru6P to form fructose 1, 6-bisphosphate. PFK-1 is an allosteric enzyme showing cooperative behaviour with Fru6P and hyperbolic kinetics with ATP.
Contents
Chemical equation
![Fru6P + ATP \rightleftharpoons Fru1,6BP + ADP](/wiki/images/math/f/9/3/f931feed721084327fc70fe25f43784b.png)
Rate equation
The concerted transition model of Monod, Wyman and Changeux (MWC model) is used as a rate equation for this tetrameric enzyme for considering exclusive ligand binding (F6P, activators and inhibitors) together with mixed type activation, (Fru2,6BP or AMP or Pi) [1].
Parameter values
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
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0.031 [1] | ![]() |
HeLa cell line | Moreno-Sánchez, Marín-Hernández, Encalada & Saavedra, unpublished results |
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1.0 [1] | mM | ||
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0.021[1] | mM | ||
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20[1] | mM | ||
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6.8[1] | mM | ||
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mM | ||
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0.32[1] | Dimensionless | ||
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0.98[1] | Dimensionless | ||
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4.1[1] | Dimensionless | ||
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5[1] | mM | ||
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5[1] | mM | ||
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247[1] | mM | Recalculated from the ![]() |
Parameters with uncertainty
- The
and
in the rate equation represents the factors by which the ligand affinity and catalytic capacity are modified in the presence of an allosteric activatory [2]. As
is the only activator in our model we considered the
and
value of
,
and
. These two values are measured in the presence of 140
medium [2].
- The Vm value is reported as Failed to parse (Cannot store math image on filesystem.): 56 \pm 23
(nmol/min/mg protein). HeLa cells were harvested at a concentration of 65 mg protein/ml [2]. Converting Vm to mM/min becomes Failed to parse (Cannot store math image on filesystem.): 0.00364 \pm 0.001495
again in the presence of 140
.
- Four Keq values have been reported in the SilicoTrypWiki (Wikipedia for insilico modelling of Trypanosome) for Phosphofructokinase: 308.4, 254, 1035, 800. Taking the mean and std. dev. of this value is
[3]
Parameter | Value | Units |
---|---|---|
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Failed to parse (Cannot store math image on filesystem.): 0.00364 \pm 0.001495 | ![]() |
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mM |
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0.021[1] | mM |
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20[1] | mM |
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mM |
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mM |
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Dimensionless |
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Dimensionless |
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Dimensionless |
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5[1] | mM |
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5[1] | mM |
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References
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011). Modeling cancer glycolysis. Biochim Biophys Acta, 1807:755–767 (doi)
- ↑ 2.0 2.1 2.2 R. Moreno-Sánchez, A. Marín-Hernández, J.C. Gallardo-Pérez, H. Quezada, R. Encalada, S. Rodríguez-Enríquez et al. (2012), Phosphofructokinase type 1 kinetics, isoform expression, and gene polymorphisms in cancer cells, J Cell Biochem, 113, pp. 1692–1703
- ↑ [[1]]