Difference between revisions of "Transformation of PGH2 to PGE2"

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(PGES Enzyme Parameters)
(PGES Enzyme Parameters)
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|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
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{|class="wikitable sortable"
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|+  style="text-align: left;" | Gibbs Free Energy Change
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|-
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! Value
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! Units
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! Species
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! Notes
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! Reference
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|-
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|5.72
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|kcal/mol
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|Not stated
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|Estimated
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Enzyme: PGES
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Substrate: Arachidonate
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Product: PGE2
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pH: 7.3
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ionic strength: 0.25
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|<ref name="MetaCyc”>[http://metacyc.org/META/NEW-IMAGE?type=REACTION&object=PROSTAGLANDIN-E-SYNTHASE-RXN Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471]</ref>
 
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Revision as of 14:30, 18 August 2016

Return to overview

Reaction

R10 PGH2 - PGE2.jpg

Chemical equation

 FA \rightleftharpoons AA

Rate equation

PGES Enzyme Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
1.6E-01 ± 4.00E-03  mM Human Expression Vector: E. Coli

Enzyme: PGES pH: 8 Temperature: 37

[1]
2.15E-01  mM Human Wild Type Enzyme [2]
1.49E-02  mM Human Expression Vector: E. Coli

Enzyme: PGES pH: Unknown Temperature: Unknown

Other: cPGES, casein kinase II and Hsp90

[3]
6.66E-02  mM Human Expression Vector: E. Coli

Enzyme: PGES pH: Unknown Temperature: Unknown

[3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
3000 ± 360 per minute Human Expression Vector: E. Coli.

Enzyme: Microsomal Prostaglandin E Synthase pH: 7.5 Temperature: 37

[4]
PGES Abundance
Value Units Species Notes Reference
220  ppm Human Expression Vector: Placenta

Enzyme: PGES pH: 7.5 Temperature: 37 °C

[5]
75.3  ppm Human Expression Vector: Urinary Bladder

Enzyme: PGES pH: 7.5 Temperature: 37 °C

[6]
208  ppm Human Expression Vector: Stomach

Enzyme: PGES pH: 7.5 Temperature: 37 °C

[5]
28.1  ppm Human Expression Vector: Lung

Enzyme: PGES pH: 7.5 Temperature: 37 °C

[6]
11.6  ppm Human Expression Vector: Colon

Enzyme: PGES pH: 7.5 Temperature: 37 °C

[6]
Gibbs Free Energy Change
Value Units Species Notes Reference
5.72 kcal/mol Not stated Estimated

Enzyme: PGES Substrate: Arachidonate Product: PGE2 pH: 7.3 ionic strength: 0.25

[7]

References


Related Reactions