Difference between revisions of "Transformation of 12-HPETE to ex12-HPETE"

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(Transport Parameters)
(Transporter concentration)
 
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=== Transporter concentration ===
 
=== Transporter concentration ===
 +
 +
To convert the enzyme concentration from ppm to mM, the following [[Common equations#Enzyme concentration (mM)|equation]] was used.
 +
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
 
|+  style="text-align: left;" | Literature values
 
|+  style="text-align: left;" | Literature values
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{| class="wikitable"
 
{| class="wikitable"
 
|+  style="text-align: left;" | Description of the ABC concentration distribution
 
|+  style="text-align: left;" | Description of the ABC concentration distribution
! Mode (mM) !! Confidence Interval  !! Location parameter (µ) !! Scale parameter (σ)
+
! Mode (ppm) !! Mode (mM) !! Confidence Interval  !! Location parameter (µ) !! Scale parameter (σ)
 
|-
 
|-
| 5.06E+00 || 1.44E+00 || 1.74E+00 || 3.42E-01
+
| 5.06E+00 || 2.80E-05 || 1.44E+00 || 1.74E+00 || 3.42E-01
 
|}
 
|}
  

Latest revision as of 11:11, 30 May 2019

Return to overview

Return to ATP-Binding Cassette Transporters


Rate equation

R37.PNG

Transport Parameters

Kms

Literature values
Value Units Species Notes Weight Reference
0.0109 ± 0.0041 mM Human Substrate: LTC4

pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes.

512 [1]
0.0000366 ± 0.0000038 mM Human Substrate LTC4, 37 °C, 4 mM ATP, 128 [2]
5.3E-3 ± 2.6E-3 mM Human 4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30

mg protein).

128 [3]
0.1954 ± 0.0612 mM Human pH 7.0, 37°C, recombinant MRP2,

Substrate: 17beta-estradiol 17-(beta-D-glucuronide)

512 [1]
0.1097 ± 0.0391 mM Human pH 7.0, 37°C, recombinant MRP2,

Substrate: Estrone glucuronide

512 [1]
0.0207 mM Human pH 7.4, 37°C, Substrate: Progesterone, Expression vector: Human HepG2 hepatoma 2048 [4]
Description of the ABC Kms distribution
Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
1.13E+01 1.00E+01 3.22E+00 8.91E-01
The estimated probability distribution for ABC Kms. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

kcat

No data available on the turnover of ABCC4 transporter therefore based upon estimates made by http://book.bionumbers.org/what-are-the-rates-of-membrane-transporters/

Literature values
Value Units Transporter Notes Weight Reference
3.5 min-1 Glucose transporter ptsI Organism: Bacteria Escherichia coli

Back of the envelope calculation by BioNumbers: http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=102931

8 [5]
0.33 - 0.83 min-1 Rate of transport of H+/Lactose transporter Organism: Bacteria Escherichia coli

Back of the envelop calculation on bionumbers http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=103159

8 [6]
3.28 min-1 Catalytic Rate of transporter HXT7 Organism: Budding yeast Saccharomyces cerevisiae

Back of the envelope calculation http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=101737

8 [7]
11.34 min-1 Human pH 7.0, 37°C, recombinant MRP2,

Substrate: Estrone glucuronide

8 [1]
Description of the ABC kcat distribution
Mode (min-1) Confidence Interval Location parameter (µ) Scale parameter (σ)
2.10E-02 2.37E+01 -2.60E+00 1.12E+00
The estimated probability distribution for ABC kcat. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

Vmax

Literature values
Value Units Species Notes Reference
0.00019 ± 1.96e-5 mmol/min/mg protein Human Substrate: LTC4

pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes.

[1]
1.25e-7 ± 1.2e-8 mmol/min/mg Human Substrate LTC4, 37 °C, 4 mM ATP, [2]
2.02e-8 ± 5.9e-9 mmol/mg/min Human 4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30

mg protein).

[3]

Transporter concentration

To convert the enzyme concentration from ppm to mM, the following equation was used.

Literature values
Value Units Species Notes Weight Reference
5.15 ppm Human Expression Vector: Stomach

Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C

2048 [8]
5.94 ppm Human Expression Vector: Lung

Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C

2048 [9]
2.66 ppm Human Expression Vector: Gut

Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C

2048 [9]
Description of the ABC concentration distribution
Mode (ppm) Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
5.06E+00 2.80E-05 1.44E+00 1.74E+00 3.42E-01
The estimated probability distribution for ABC concentration. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

References