Difference between revisions of "Transformation of 12-HPETE to ex12-HPETE"
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=== Transporter concentration === | === Transporter concentration === | ||
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+ | To convert the enzyme concentration from ppm to mM, the following [[Common equations#Enzyme concentration (mM)|equation]] was used. | ||
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{|class="wikitable sortable" | {|class="wikitable sortable" | ||
|+ style="text-align: left;" | Literature values | |+ style="text-align: left;" | Literature values | ||
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{| class="wikitable" | {| class="wikitable" | ||
|+ style="text-align: left;" | Description of the ABC concentration distribution | |+ style="text-align: left;" | Description of the ABC concentration distribution | ||
− | ! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ) | + | ! Mode (ppm) !! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ) |
|- | |- | ||
− | | 5.06E+00 || 1.44E+00 || 1.74E+00 || 3.42E-01 | + | | 5.06E+00 || 2.80E-05 || 1.44E+00 || 1.74E+00 || 3.42E-01 |
|} | |} | ||
Latest revision as of 11:11, 30 May 2019
Return to ATP-Binding Cassette Transporters
Contents
Rate equation
Transport Parameters
Kms
Value | Units | Species | Notes | Weight | Reference |
---|---|---|---|---|---|
0.0109 ± 0.0041 | mM | Human | Substrate: LTC4
pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes. |
512 | [1] |
0.0000366 ± 0.0000038 | mM | Human | Substrate LTC4, 37 °C, 4 mM ATP, | 128 | [2] |
5.3E-3 ± 2.6E-3 | mM | Human | 4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30
mg protein). |
128 | [3] |
0.1954 ± 0.0612 | mM | Human | pH 7.0, 37°C, recombinant MRP2,
Substrate: 17beta-estradiol 17-(beta-D-glucuronide) |
512 | [1] |
0.1097 ± 0.0391 | mM | Human | pH 7.0, 37°C, recombinant MRP2,
Substrate: Estrone glucuronide |
512 | [1] |
0.0207 | mM | Human | pH 7.4, 37°C, Substrate: Progesterone, Expression vector: Human HepG2 hepatoma | 2048 | [4] |
Mode (mM) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|
1.13E+01 | 1.00E+01 | 3.22E+00 | 8.91E-01 |
kcat
No data available on the turnover of ABCC4 transporter therefore based upon estimates made by http://book.bionumbers.org/what-are-the-rates-of-membrane-transporters/
Value | Units | Transporter | Notes | Weight | Reference |
---|---|---|---|---|---|
3.5 | min-1 | Glucose transporter ptsI | Organism: Bacteria Escherichia coli
Back of the envelope calculation by BioNumbers: http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=102931 |
8 | [5] |
0.33 - 0.83 | min-1 | Rate of transport of H+/Lactose transporter | Organism: Bacteria Escherichia coli
Back of the envelop calculation on bionumbers http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=103159 |
8 | [6] |
3.28 | min-1 | Catalytic Rate of transporter HXT7 | Organism: Budding yeast Saccharomyces cerevisiae
Back of the envelope calculation http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=101737 |
8 | [7] |
11.34 | min-1 | Human | pH 7.0, 37°C, recombinant MRP2,
Substrate: Estrone glucuronide |
8 | [1] |
Mode (min-1) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|
2.10E-02 | 2.37E+01 | -2.60E+00 | 1.12E+00 |
Vmax
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
0.00019 ± 1.96e-5 | mmol/min/mg protein | Human | Substrate: LTC4
pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes. |
[1] |
1.25e-7 ± 1.2e-8 | mmol/min/mg | Human | Substrate LTC4, 37 °C, 4 mM ATP, | [2] |
2.02e-8 ± 5.9e-9 | mmol/mg/min | Human | 4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30
mg protein). |
[3] |
Transporter concentration
To convert the enzyme concentration from ppm to mM, the following equation was used.
Value | Units | Species | Notes | Weight | Reference |
---|---|---|---|---|---|
5.15 | ppm | Human | Expression Vector: Stomach
Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C |
2048 | [8] |
5.94 | ppm | Human | Expression Vector: Lung
Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C |
2048 | [9] |
2.66 | ppm | Human | Expression Vector: Gut
Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C |
2048 | [9] |
Mode (ppm) | Mode (mM) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|---|
5.06E+00 | 2.80E-05 | 1.44E+00 | 1.74E+00 | 3.42E-01 |
References
- ↑ 1.0 1.1 1.2 1.3 1.4 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2) Eur. J. Pharm. Sci. 35, 326-334 (2008)
- ↑ 2.0 2.1 Zeng H. "Transport of amphipathic anions by human multidrug resistance protein 3 J. Biol. Chem. 275, 34166-34172 (2000) Cite error: Invalid
<ref>
tag; name "Mao2000" defined multiple times with different content - ↑ 3.0 3.1 "Functional reconstitution of substrate transport by purified multidrug resistance protein MRP1 (ABCC1) in phospholipid vesicles Cancer Res. 60, 4779-4784 (2000)
- ↑ W. Fong "Schisandrol A from Schisandra chinensis Reverses P-Glycoprotein-Mediated Multidrug Resistance by Affecting Pgp-Substrate Complexes Planta Med 2007; 73(3): 212-220
- ↑ Waygood EB Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties.Can J Biochem. 1980 Jan;58(1):40-8.
- ↑ Naftalin R. J. ELactose permease H+-lactose symporter: mechanical switch or Brownian ratchet?Biophys J. 2007 May 15;92(10):3474-91. Epub 2007 Feb 26.
- ↑ Ye L. Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiaeYeast. 2001 Sep 30;18(13):1257-67.
- ↑ M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
- ↑ 9.0 9.1 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581