Difference between revisions of "Transformation of PGH2 to PGD2"

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(Parameters)
(Parameters)
Line 23: Line 23:
 
|<math> mM </math>
 
|<math> mM </math>
 
|Human  
 
|Human  
|Cerebrospinal Fluid
+
|Expression Vector: Human Cell
|<ref name="Watanabe1994"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Watanabe K. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6.]</ref>
+
Enzyme: PGDS
 +
pH: 8
 +
Temperature: 25
 +
|<ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28.
 +
]</ref>
 
|-
 
|-
 
|4.00E-03
 
|4.00E-03
 
|<math> mM </math>
 
|<math> mM </math>
 
|Human
 
|Human
|Human Wild Type Enzyme
+
|Expression Vector: Cerebrospinal Fluid
|<ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/8093029 Zhou Y. , "Identification of beta-trace as prostaglandin D synthase.'' FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28.]</ref>   
+
Enzyme: PGDS
 +
pH:10
 +
Temperature: Unspecified
 +
 +
|<ref name="Watanabe1994"> [http://www.ncbi.nlm.nih.gov/pubmed/8093029 Watanabe K. , "Identification of beta-trace as prostaglandin D synthase.''
 +
Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6. ]</ref>   
 
|-
 
|-
  
Line 63: Line 72:
 
|mM
 
|mM
 
|Human
 
|Human
|20 ug/3ml of a 26 kDa protein. Cerebral Fluid.
+
Expression Vector: Cerebrospinal Fluid
 +
Enzyme: PGDS
 +
pH:10
 +
Temperature: Unspecified
 +
 
 +
Calculated:
 +
20 ug/3ml of a 26 kDa protein.
 
|<ref name="Watanabe1994"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Watanabe K. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6.]</ref>
 
|<ref name="Watanabe1994"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Watanabe K. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6.]</ref>
 
|-
 
|-
Line 69: Line 84:
 
|mM
 
|mM
 
|Human
 
|Human
|20mg/ml of 26 kDa protein
+
|Expression Vector: Human Cell
 +
Enzyme: PGDS
 +
pH: 8
 +
Temperature: 25
 +
 
 +
Calculated: 20mg/ml of 26 kDa protein
 
|<ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' FASEB J. 2010 Dec;24(12):4668-77.]</ref>  
 
|<ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' FASEB J. 2010 Dec;24(12):4668-77.]</ref>  
 
|-
 
|-

Revision as of 13:24, 28 May 2016

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R21 PGH2 - PGD2.jpg

Chemical equation

 FA \rightleftharpoons AA

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
4.00E-03  mM Human Expression Vector: Human Cell

Enzyme: PGDS pH: 8 Temperature: 25

[1]
4.00E-03  mM Human Expression Vector: Cerebrospinal Fluid

Enzyme: PGDS pH:10 Temperature: Unspecified

[2]
Enzyme Turnover Numbers
Value Units Species Notes Reference
158.4 per minute Human Wild type (Paper includes 13 mutants of the enzyme) [1]
Enzyme Concentrations
Value Units Species Notes Reference
0.000256 mM Human

Expression Vector: Cerebrospinal Fluid Enzyme: PGDS pH:10 Temperature: Unspecified

Calculated: 20 ug/3ml of a 26 kDa protein.

[2]
0.769 mM Human Expression Vector: Human Cell

Enzyme: PGDS pH: 8 Temperature: 25

Calculated: 20mg/ml of 26 kDa protein

[1]

References

  1. 1.0 1.1 1.2 [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules. FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28. ] Cite error: Invalid <ref> tag; name "Zhou2010" defined multiple times with different content Cite error: Invalid <ref> tag; name "Zhou2010" defined multiple times with different content
  2. 2.0 2.1 [http://www.ncbi.nlm.nih.gov/pubmed/8093029 Watanabe K. , "Identification of beta-trace as prostaglandin D synthase. Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6. ] Cite error: Invalid <ref> tag; name "Watanabe1994" defined multiple times with different content

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