Transformation of AA to 20-HETE

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Reaction

Chemical equation

AA \rightleftharpoons 20-HETE

Rate equation

CYP Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
0.0118 mM Bacillus licheniformis Expression Vector: Bacillus licheniformis

Substrate: Stearic acid Enzyme: CYP102A7 pH: 7.5 Temperature: Unspecified

[1]
0.0551 mM Bacillus licheniformis Expression Vector: Bacillus licheniformis

Substrate: Linoleic acid Enzyme: CYP102A7 pH: 7.5 Temperature: Unspecified

[1]
0.0165 mM Bacillus licheniformis Expression Vector: Bacillus licheniformis

Substrate: Palmitoleic acid Enzyme: CYP102A7 pH: 7.5 Temperature: Unspecified

[1]
0.008 mM Human Expression Vector: Human

Substrate: Fenthion Enzyme: CYP1A1 pH: Unspecified Temperature: 37

[2]
0.0044 mM Human Expression Vector: Human

Substrate: Fenthion Enzyme: CYP2C19 pH: Unspecified Temperature: 37

[2]
0.012 mM Human Expression Vector: Human

Substrate: Fenthion Enzyme: CYP2C9 pH: Unspecified Temperature: 37

[2]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1368 per minute Bacillus licheniformis Expression Vector: Bacillus licheniformis

Substrate: Palmitoleic acid Enzyme: CYP102A7 pH: 7.5 Temperature: Unspecified

[1]
1008 per minute Bacillus licheniformis Expression Vector: Bacillus licheniformis

Substrate: stearic acid Enzyme: CYP102A7 pH: 7.5 Temperature: Unspecified

[1]
3588 per minute Bacillus licheniformis Expression Vector: Bacillus licheniformis

Substrate: tetradecanoic acid Enzyme: CYP102A7 pH: 7.5 Temperature: Unspecified

[1]
1758 per minute Bacillus licheniformis Expression Vector: Bacillus licheniformis

Substrate: linoleic acid Enzyme: CYP102A7 pH: 7.5 Temperature: Unspecified

[1]


CYP Abundance
Value Units Species Notes Reference
6.8 ppm Human Expression Vector: Lung

Enzyme: CYP1A1 pH: 7.5 Temperature: 37 °C

[3]
1.08 ppm Human Expression Vector: Cell line metastatic pancreatic

Enzyme: CYP1A1 pH: 7.5 Temperature: 37 °C

[4]
157 ppm Human Expression Vector: Lung

Enzyme: CYP2C19 pH: 7.5 Temperature: 37 °C

[3]
2.35 ppm Human Expression Vector: Gut

Enzyme: CYP2C19 pH: 7.5 Temperature: 37 °C

[4]
0.04 ppm Human Expression Vector: Lung

Enzyme: CYP2C19 pH: 7.5 Temperature: 37 °C

[4]
Gibbs Free Energy Change
Value Units Species Notes Reference
(-40.47) kcal/mol Not stated Estimated Enzyme: CYP2C19

Substrate:melatonin Product:N-acetyl-serotonin pH: 7.3 ionic strength: 0.25

[5]
(-20.98) kcal/mol Not stated Estimated Enzyme: CYP1A2

Substrate:melatonin Product:N-acetyl-serotonin pH: 7.3 ionic strength: 0.25

[5]

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 M. Dietrich “Cloning, expression and characterisation of CYP102A7, a self-sufficient P450 monooxygenase from Bacillus licheniformis” Applied Microbiology and Biotechnology, July 2008, Volume 79, Issue 6, pp 931–940
  2. ↑ 2.0 2.1 2.2 Leonari C. “The participation of human hepatic P450 isoforms, flavin-containing monooxygenases and aldehyde oxidase in the biotransformation of the insecticide fenthion” Toxicology and Applied Pharmacology, Volume 233, Issue 2, 1 December 2008, Pages 343-352
  3. ↑ 3.0 3.1 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
  4. ↑ 4.0 4.1 4.2 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  5. ↑ 5.0 5.1 Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471


Related Reactions

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