Difference between revisions of "UDPG-pyrophosphorylase"
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|<math>200 \pm 21</math> <ref name = "villar_1960"> Villar-Palasi C & Larner J (1960). ''Levels of activity of the enzymes of the glycogen cycle in rat tissues''. Arch Biochem Biophys 86, 270–273.</ref> | |<math>200 \pm 21</math> <ref name = "villar_1960"> Villar-Palasi C & Larner J (1960). ''Levels of activity of the enzymes of the glycogen cycle in rat tissues''. Arch Biochem Biophys 86, 270–273.</ref> | ||
− | |<math>min^{-1}</math> | + | |<math>mM min^{-1}</math> |
|rowspan="6"|Recombinant, human muscle | |rowspan="6"|Recombinant, human muscle | ||
|rowspan="6"| | |rowspan="6"| |
Latest revision as of 14:42, 22 September 2014
This enzyme converts UTP and G1P to UDP-glucose (UDPG) and pyrophosphate (PPi)
Contents
Chemical equation
Rate equation
Reversible Bi substrate Michaelis-Menten equation with random binding order is used [1]
Parameter values
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
200 [2] | Recombinant, human muscle | |||
0.4 [3] | mM | |||
0.92 [3] | mM | |||
[3] | mM | |||
[3] | mM | |||
[4] | Dimensionless |
Parameters with uncertainty
- The value of is reported to be of . The Std. Dev. for was considered to be of its mean value. Same error percentage is considered for .
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
[2] | Recombinant, human muscle | |||
[3] | mM | |||
[3] | mM | |||
[3] | mM | |||
[3] | mM | |||
Dimensionless |
Equilibrium constant
Equilibrium constant | Conditions | Source |
---|---|---|
pH=7 and 7.9, T=25°C, 10mM Mg2+ | NIST database "Thermodynamics of Enzyme-Catalyzed Reactions" entry [[1]] from Atkinson et al. (1958) [5] reported 4 values for Keq; 0.119, 0.286, 0.139, 0.263. Taking mean and std. for these values give (n=4). |
References
- ↑ Palm, D.C. (2013). The regulatory design of glycogen metabolism in mammalian skeletal muscle (Ph.D.). University of Stellenbosch
- ↑ 2.0 2.1 Villar-Palasi C & Larner J (1960). Levels of activity of the enzymes of the glycogen cycle in rat tissues. Arch Biochem Biophys 86, 270–273.
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 Duggleby RG, Chao YC, Huang JG, Peng HL & Chang HY (1996). Sequence differences between human muscle and liver cDNAs for UDPglucose pyrophosphorylase and kinetic properties of the recombinant enzymes expressed in Escherichia coli. Eur J Biochem 235, 173–179.
- ↑ Bergamini C, Signorini M, Ferrari C & Dallocchio F (1983), Non-Michaelian kinetics of rabbit muscle uridine diphosphoglucose pyrophosphorylase, Arch Biochem Biophys 227, 397–405
- ↑ Turner, D.H.; Turner, J.F.; Biochem. J.; 69, 448 (1958)