Difference between revisions of "Triosephosphate isomerase"
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==Parameters with uncertainty== | ==Parameters with uncertainty== | ||
− | * The activity is measured in Activity in the reverse reaction in Hernandez (2006) ''et. al.'' <math>V_{mf}</math> is sampled based on Haldane equation <math>K_{eq} = \frac{V_{forward}*K_{product}}{V_{reverse}*K_{substrate}}</math> using the value <math>K_{eq} = 20.9 \pm 3.1</math>, <math>Km_{Gly3P}</math> and <math>Km_{DHAP}</math>. ''' | + | * The activity is measured in Activity in the reverse reaction in Hernandez (2006) ''et. al.'' <math>V_{mf}</math> is sampled based on Haldane equation <math>K_{eq} = \frac{V_{forward}*K_{product}}{V_{reverse}*K_{substrate}}</math> using the value <math>K_{eq} = 20.9 \pm 3.1</math>, <math>Km_{Gly3P}</math> and <math>Km_{DHAP}</math>. <br> |
+ | '''Alternative-1''' the reported fixed point value can be considered with the standard deviation calculated based on the same ratio of <math>V_{mf}</math> which is <math>\approx 31%</math>. This gives the value <math>V_{mf}=5 \pm 1.55 </math><br> | ||
+ | '''Alternative-2''' Calculating <math>V_{mf}</math> from <math>V_{mr}</math> based on Haldane equation which gives the value of 2.911 and with the same percent of erro Std. Dev. is 0.90. | ||
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|<math>V_{mf}</math> | |<math>V_{mf}</math> | ||
− | | Sampled based on the Haldane equation.<br> '''Alternative:''' <math>5 \pm 1.55</math> | + | | Sampled based on the Haldane equation.<br> '''Alternative:''' <math>5 \pm 1.55</math> or <math>2.911 \pm 0.90</math> |
|<math> mM \times min^{-1} </math> | |<math> mM \times min^{-1} </math> | ||
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Revision as of 19:30, 24 June 2014
This enzyme rapidly inter-converts the molecules Dihydroxyacetone phosphate (DHAP) and Glyceraldehyde 3-phosphate (Gly3P). Gly3P is removed as soon as it is formed to be used in the next step of glycolysis.
Chemical equation
Rate equation
Reversible Michaelis-Menten is used [1]
Modified rate law considering thermodynamic constant is
Paramters
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
5 [1] | Hela cell line | |||
42[2] | ||||
0.51[1] | mM | |||
1.6[1] | mM |
Parameters with uncertainty
- The activity is measured in Activity in the reverse reaction in Hernandez (2006) et. al. is sampled based on Haldane equation using the value Failed to parse (Cannot store math image on filesystem.): K_{eq} = 20.9 \pm 3.1
, and .
Alternative-1 the reported fixed point value can be considered with the standard deviation calculated based on the same ratio of which is . This gives the value Failed to parse (Cannot store math image on filesystem.): V_{mf}=5 \pm 1.55
Alternative-2 Calculating from based on Haldane equation which gives the value of 2.911 and with the same percent of erro Std. Dev. is 0.90.
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
Sampled based on the Haldane equation. Alternative: Failed to parse (Cannot store math image on filesystem.): 5 \pm 1.55 or |
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[2] | ||||
[3] | mM | Human liver | ||
[3] | mM | Human liver | ||
[3] | mM |
References
- ↑ 1.0 1.1 1.2 1.3 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi) Cite error: Invalid
<ref>
tag; name "Hernandez2011" defined multiple times with different content - ↑ 2.0 2.1 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
- ↑ 3.0 3.1 3.2 Snyder, R.; Lee, E.W. (1975), Triosephosphate isomerase from human and horse liver,Methods Enzymol. 41B, 430-434