Difference between revisions of "Transformation of TXA2 to exTXA2 by PGT"

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(→‎Transport Parameters)
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! Species
 
! Species
 
! Notes
 
! Notes
 +
! Weight
 
! Reference
 
! Reference
 
|-
 
|-
Line 25: Line 26:
 
pH: 7.5
 
pH: 7.5
 
Temp: 37 °C
 
Temp: 37 °C
 +
|2048
 
|<ref name="Kraft2010"> [https://www.ncbi.nlm.nih.gov/pubmed/20019365 M. Kraft ''The prostaglandin transporter OATP2A1 is expressed in human ocular tissues and transports the antiglaucoma prostanoid latanoprost.'' Invest Ophthalmol Vis Sci. 2010 May;51(5):2504-11. doi: 10.1167/iovs.09-4290. Epub 2009 Dec 17.]</ref>
 
|<ref name="Kraft2010"> [https://www.ncbi.nlm.nih.gov/pubmed/20019365 M. Kraft ''The prostaglandin transporter OATP2A1 is expressed in human ocular tissues and transports the antiglaucoma prostanoid latanoprost.'' Invest Ophthalmol Vis Sci. 2010 May;51(5):2504-11. doi: 10.1167/iovs.09-4290. Epub 2009 Dec 17.]</ref>
 
|-
 
|-
Line 33: Line 35:
 
pH: unknown
 
pH: unknown
 
Temp: 37 °C
 
Temp: 37 °C
 +
|512
 
|<ref name="Gose2016"> [https://www.ncbi.nlm.nih.gov/pubmed/26692285 Gose T. ''Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3.'' Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.]</ref>
 
|<ref name="Gose2016"> [https://www.ncbi.nlm.nih.gov/pubmed/26692285 Gose T. ''Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3.'' Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.]</ref>
 
|-
 
|-
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pH: unknown
 
pH: unknown
 
Temp: 37 °C
 
Temp: 37 °C
 +
|512
 
|<ref name="Gose2016"> [https://www.ncbi.nlm.nih.gov/pubmed/26692285 Gose T. ''Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3.'' Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.]</ref>
 
|<ref name="Gose2016"> [https://www.ncbi.nlm.nih.gov/pubmed/26692285 Gose T. ''Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3.'' Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.]</ref>
 
|-
 
|-
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pH: unknown
 
pH: unknown
 
Temp: unknown
 
Temp: unknown
 +
|192
 
|<ref name="Chi2010"> [http://www.sciencedirect.com/science/article/pii/S0006291X10005723 Chi Y. ''The prostaglandin transporter PGT transports PGH2'' Biochemical and Biophysical Research Communications, Volume 395, Issue 2, 30 April 2010, Pages 168–172]</ref>
 
|<ref name="Chi2010"> [http://www.sciencedirect.com/science/article/pii/S0006291X10005723 Chi Y. ''The prostaglandin transporter PGT transports PGH2'' Biochemical and Biophysical Research Communications, Volume 395, Issue 2, 30 April 2010, Pages 168–172]</ref>
 
|-
 
|-
Line 59: Line 64:
 
pH: 7
 
pH: 7
 
Temperature: 27
 
Temperature: 27
 +
|1024
 
|<ref name="Kanai1995"> [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. ''Identification and Characterization of a
 
|<ref name="Kanai1995"> [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. ''Identification and Characterization of a
 
Prostaglandin Transporter'' Science  12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]</ref>
 
Prostaglandin Transporter'' Science  12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]</ref>
Line 70: Line 76:
 
pH: 7
 
pH: 7
 
Temperature: 27
 
Temperature: 27
 +
|1024
 
|<ref name="Kanai1995"> [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. ''Identification and Characterization of a
 
|<ref name="Kanai1995"> [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. ''Identification and Characterization of a
 
Prostaglandin Transporter'' Science  12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]</ref>
 
Prostaglandin Transporter'' Science  12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]</ref>
Line 81: Line 88:
 
pH: 7
 
pH: 7
 
Temperature: 27
 
Temperature: 27
 +
|1024
 
|<ref name="Kanai1995"> [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. ''Identification and Characterization of a
 
|<ref name="Kanai1995"> [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. ''Identification and Characterization of a
 
Prostaglandin Transporter'' Science  12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]</ref>
 
Prostaglandin Transporter'' Science  12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]</ref>
Line 92: Line 100:
 
pH: 7
 
pH: 7
 
Temperature: 27
 
Temperature: 27
 +
|1024
 
|<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
 
|<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
−
|-
 
−
|5.02E-05
 
−
|mM
 
−
|Human
 
−
|Substrate: PGF2a
 
−
Species: Human
 
−
Vector: HeLa Cell Line
 
−
pH: 7
 
−
Temperature: 27
 
−
|<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
 
−
|-
 
−
|0.005
 
−
|mM
 
−
|Human
 
−
|Substrate: 15-Keto-PGE2
 
−
Species: Human
 
−
Vector: HeLa Cell Line
 
−
pH: 7
 
−
Temperature: 27
 
−
|<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
 
−
|-
 
−
|0.005
 
−
|mM
 
−
|Human
 
−
|Substrate: 13,14-Dihydo-15-Keto-PGE2
 
−
Species: Human
 
−
Vector: HeLa Cell Line
 
−
pH: 7
 
−
Temperature: 27
 
−
|<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
 
−
|-
 
−
|3.82E-05
 
−
|mM
 
−
|Human
 
−
|Substrate: PGH2
 
−
Species: Human
 
−
Vector: HeLa Cell Line
 
−
pH: 7
 
−
Temperature: 27
 
−
|<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
 
−
|-
 
−
|}
 
−
 
−
{| class="wikitable"
 
−
|+  style="text-align: left;" | Description of the PGT Kms distribution
 
−
! Mode (mM) !! Confidence Interval  !! Location parameter (µ) !! Scale parameter (σ)
 
−
|-
 
−
| 1.10E-03 || 9.42E+01 || -4.67E+00 || 1.45E+00
 
−
|}
 
−
 
−
[[Image:142.jpg|none|thumb|500px|The estimated probability distribution for PGT Kms. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.  ]]
 
−
 
−
===k<sub>cat</sub>===
 
−
No data available on the turnover of PGT/ an organic molecule transporter therefore based upon estimates made by http://book.bionumbers.org/what-are-the-rates-of-membrane-transporters/
 
−
{|class="wikitable sortable"
 
−
|+  style="text-align: left;" | Literature values
 
−
|-
 
−
! Value
 
−
! Units
 
−
! Transporter
 
−
! Notes
 
−
! Reference
 
−
|-
 
−
|3.5
 
−
|min-1
 
−
|Glucose transporter ptsI
 
−
|Organism: Bacteria Escherichia coli
 
−
Back of the envelope calculation by BioNumbers: http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=102931
 
−
|<ref name="Waygood1980"> [https://www.ncbi.nlm.nih.gov/pubmed/6992959?dopt=Abstract Waygood EB ''Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties.''Can J Biochem. 1980 Jan;58(1):40-8.]</ref>
 
−
|-
 
−
|0.33 - 0.83
 
−
|min-1
 
−
|Rate of transport of H+/Lactose transporter
 
−
|Organism: Bacteria Escherichia coli
 
−
Back of the envelop calculation on bionumbers http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=103159
 
−
|<ref name="Naftalin2007"> [https://www.ncbi.nlm.nih.gov/pubmed/17325012?dopt=Abstract Naftalin R. J. ''ELactose permease H+-lactose symporter: mechanical switch or Brownian ratchet?''Biophys J. 2007 May 15;92(10):3474-91. Epub 2007 Feb 26.]</ref>
 
−
|-
 
−
|3.28
 
−
|min-1
 
−
|Catalytic Rate of transporter HXT7
 
−
|Organism: Budding yeast Saccharomyces cerevisiae
 
−
Back of the envelope calculation http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=101737
 
−
|<ref name="Ye2001"> [https://www.ncbi.nlm.nih.gov/pubmed/11561293?dopt=Abstract Ye L. ''Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiae''Yeast. 2001 Sep 30;18(13):1257-67.]</ref>
 
−
|-
 
−
|}
 
−
 
−
{| class="wikitable"
 
−
|+  style="text-align: left;" | Description of the PGT kcat distribution
 
−
! Mode (min-1) !! Confidence Interval  !! Location parameter (µ) !! Scale parameter (σ)
 
−
|-
 
−
| 1.33E+00 || 7.30E+00 || 9.20E-01 || 8.00E-01
 
−
|}
 
−
 
−
[[Image:132.jpg|none|thumb|500px|The estimated probability distribution for PGT kcat. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.  ]]
 
−
 
−
=== Transporter concentration ===
 
−
{|class="wikitable sortable"
 
−
|+  style="text-align: left;" | Literature values
 
−
|-
 
−
! Value
 
−
! Units
 
−
! Species
 
−
! Notes
 
−
! Reference
 
−
|-
 
−
|125
 
−
|ppm
 
−
|Human
 
−
|Vector:Lung
 
−
Enzyme: SLCO2A1
 
−
pH: 7.5
 
−
Temperature: 37 °C
 
−
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
−
|-
 
−
|17.4
 
−
|ppm
 
−
|Human
 
−
|Expression Vector: Esophagus
 
−
Enzyme: SLCO2A1
 
−
pH: 7.5
 
−
Temperature: 37 °C
 
−
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 
−
|-
 
−
|2.82
 
−
|ppm
 
−
|Human
 
−
|Vector:Gut
 
−
Enzyme: SLCO2A1
 
−
pH: 7.5
 
−
Temperature: 37 °C
 
−
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
 
|-
 
|-
 
|}
 
|}

Revision as of 16:21, 22 May 2019

Return to overview

Prostaglandin Transporter


Rate equation

R110.PNG

Transport Parameters

Kms

Literature values
Value Units Species Notes Weight Reference
0.0054 mM Human Eye Vector: HEK-OATP2A1 or HEK-OATP2B1 and the respective vector controls

Substrate: Latanoprost, a prostaglandin F2a analogue pH: 7.5 Temp: 37 °C

2048 [1]
0.000331 ± 0.000131 mM Transfected Human embryonic kidney cells (HEK293) Substrate: PGE2

pH: unknown Temp: 37 °C

512 [2]
0.007202 ± 0.000595 mM Transfected Human embryonic kidney cells (HEK293) Substrate: PGE3

pH: unknown Temp: 37 °C

512 [2]
0.000376 ± 3.4e-5 mM PGT-expressing Madin-Darby Canine Kidney Epithelial Cells (MDCK) cells Substrate: PGH2

pH: unknown Temp: unknown

192 [3]
9.40E-05 mM Human Substrate: PGE2

Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27

1024 [4]
0.000423 mM Human Substrate: TXB2

Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27

1024 [4]
0.007569 mM Human Substrate: 6-Keto-PGF1a

Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27

1024 [4]
4.87E-05 mM Human Substrate: PGE2

Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27

1024 [5]
Description of the PGT concentration distribution
Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
1.73E+01 4.72E+00 3.90E+00 1.02E+00
The estimated probability distribution for PGT concentration. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

References

  1. ↑ M. Kraft The prostaglandin transporter OATP2A1 is expressed in human ocular tissues and transports the antiglaucoma prostanoid latanoprost. Invest Ophthalmol Vis Sci. 2010 May;51(5):2504-11. doi: 10.1167/iovs.09-4290. Epub 2009 Dec 17.
  2. ↑ 2.0 2.1 Gose T. Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3. Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.
  3. ↑ Chi Y. The prostaglandin transporter PGT transports PGH2 Biochemical and Biophysical Research Communications, Volume 395, Issue 2, 30 April 2010, Pages 168–172
  4. ↑ 4.0 4.1 4.2 [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. Identification and Characterization of a Prostaglandin Transporter Science 12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]
  5. ↑ Shigekazu I. Structural Determinants of Substrates for the Prostaglandin Transporter PGT Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736
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