Transformation of PGH2 to TXA2

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Reaction

R4 PGH2 -TXA4.jpg

Chemical equation

FA \rightleftharpoons AA

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
4.00E-03 mM Human Expression Vector: Human Monocyte

Enzyme: Platelet Free Human Monocyte pH: 7.4 Temperature: 37

[1]
1.00E-02 mM Human Expression Vector: Human mirosome

Enzyme: Human Thromboxane Synthase pH:7.4 Temperature:

[2]
2.20E-02 mM Human Expression Vector:Human platelet

Enzyme: Human thromboxane synthase pH: 7.4 Temperature: 37

[3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
2687 per minute Human Expression Vector: Human Platelets

Enzyme: TXAS pH: 7.4 Temperature:30

[4]
1628 per minute Human Expression Vector:Platelet Microsomes

Enzyme: TXAS pH:7.4 Temperature:37

[5]
TXAS Abundance
Value Units Species Notes Reference
949 ppm Human Expression Vector: Platelet

Enzyme: TXAS pH: 7.5 Temperature: 37 °C

[6]
56.2 ppm Human Expression Vector: Lung

Enzyme: TXAS pH: 7.5 Temperature: 37 °C

[6]
4.58 ppm Human Expression Vector: Urinary Bladder

Enzyme: TXAS pH: 7.5 Temperature: 37 °C

[6]
101 ppm Human Expression Vector: Oral Cavity

Enzyme: TXAS pH: 7.5 Temperature: 37 °C

[7]
Gibbs Free Energy Change
Value Units Species Notes Reference
41.08 kcal/mol Not stated Estimated

Enzyme: TXAS Substrate: Arachidonate Product: TXA2 pH: 7.3 ionic strength: 0.25

[8]

References

  1. ↑ M. Orlandi, G. Bartolini, Thromboxane A2 synthase activity in platelet free human monocytes. Biochim Biophys Acta. 1994 Dec 8;1215(3):285-90.
  2. ↑ R. Nusing, S. Schneider-Voss and V. Ullrich, Immunoaffinity purification of human thromboxane synthase. Arch Biochem Biophys. 1990 Aug 1;280(2):325-30.
  3. ↑ Hecker M. On the mechanism of prostacyclin and thromboxane A2 biosynthesis.J Biol Chem. 1989 Jan 5;264(1):141-50.
  4. ↑ [www.ncbi.nlm.nih.gov/pubmed/3579292 Hecker M. “Products, kinetics, and substrate specificity of homogeneous thromboxane synthase from human platelets: Development of a novel enzyme assay ” Arch. Biochem. Biophys. 1987, 254, 124-135]
  5. ↑ [www.ncbi.nlm.nih.gov/pubmed/2999104 Haurand M. “Isolation and characterization of thromboxane synthase from human platelets as a cytochrome P-450 enzyme” J Biol Chem. 1985, 260 (5), 15059-15067]
  6. ↑ 6.0 6.1 6.2 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  7. ↑ M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
  8. ↑ Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471

Related Reactions

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