Difference between revisions of "Transformation of PGH2 to TXA2"
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|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | |Platelet Free Human Monocyte | + | |Expression Vector: Human Monocyte |
| + | Enzyme: Platelet Free Human Monocyte | ||
| + | pH: 7.4 | ||
| + | Temperature: 37 | ||
|<ref name="Orlandi1994"> [http://www.ncbi.nlm.nih.gov/pubmed/7811713 M. Orlandi, G. Bartolini, Thromboxane A2 synthase activity in platelet free human monocytes.'' Biochim Biophys Acta. 1994 Dec 8;1215(3):285-90.]</ref> | |<ref name="Orlandi1994"> [http://www.ncbi.nlm.nih.gov/pubmed/7811713 M. Orlandi, G. Bartolini, Thromboxane A2 synthase activity in platelet free human monocytes.'' Biochim Biophys Acta. 1994 Dec 8;1215(3):285-90.]</ref> | ||
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| Line 31: | Line 34: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | |Human Thromboxane Synthase | + | |Expression Vector: Human mirosome |
| + | Enzyme: Human Thromboxane Synthase | ||
| + | pH:7.4 | ||
| + | Temperature: | ||
|<ref name="Nusing1989"> [http://www.ncbi.nlm.nih.gov/pubmed/2195994 R. Nusing, S. Schneider-Voss and V. Ullrich, Immunoaffinity purification of human thromboxane synthase.'' Arch Biochem Biophys. 1990 Aug 1;280(2):325-30.]</ref> | |<ref name="Nusing1989"> [http://www.ncbi.nlm.nih.gov/pubmed/2195994 R. Nusing, S. Schneider-Voss and V. Ullrich, Immunoaffinity purification of human thromboxane synthase.'' Arch Biochem Biophys. 1990 Aug 1;280(2):325-30.]</ref> | ||
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| Line 37: | Line 43: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | | | + | |Expression Vector:Human platelet |
| + | Enzyme: Human thromboxane synthase | ||
| + | pH: 7.4 | ||
| + | Temperature: 37 | ||
|<ref name="Hecker1989"> [http://www.ncbi.nlm.nih.gov/pubmed/2491846 Hecker M. ''On the mechanism of prostacyclin and thromboxane A2 biosynthesis.''J Biol Chem. 1989 Jan 5;264(1):141-50.]</ref> | |<ref name="Hecker1989"> [http://www.ncbi.nlm.nih.gov/pubmed/2491846 Hecker M. ''On the mechanism of prostacyclin and thromboxane A2 biosynthesis.''J Biol Chem. 1989 Jan 5;264(1):141-50.]</ref> | ||
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| Line 54: | Line 63: | ||
|per minute | |per minute | ||
|Human | |Human | ||
| − | |Platelets | + | |Expression Vector: Human Platelets |
| + | Enzyme: TXAS | ||
| + | pH: 7.4 | ||
| + | Temperature:30 | ||
|<ref name="Hecker1987”>[www.ncbi.nlm.nih.gov/pubmed/3579292 Hecker M. “Products, kinetics, and substrate specificity of homogeneous thromboxane synthase from human platelets: Development of a novel enzyme assay ” Arch. Biochem. Biophys. 1987, 254, 124-135]</ref> | |<ref name="Hecker1987”>[www.ncbi.nlm.nih.gov/pubmed/3579292 Hecker M. “Products, kinetics, and substrate specificity of homogeneous thromboxane synthase from human platelets: Development of a novel enzyme assay ” Arch. Biochem. Biophys. 1987, 254, 124-135]</ref> | ||
|- | |- | ||
| Line 60: | Line 72: | ||
|per minute | |per minute | ||
|Human | |Human | ||
| − | |Platelet Microsomes | + | |Expression Vector:Platelet Microsomes |
| + | Enzyme: TXAS | ||
| + | pH:7.4 | ||
| + | Temperature:37 | ||
|<ref name="Haurand1985”>[www.ncbi.nlm.nih.gov/pubmed/2999104 Haurand M. “Isolation and characterization of thromboxane synthase from human platelets as a cytochrome P-450 enzyme” J Biol Chem. 1985, 260 (5), 15059-15067]</ref> | |<ref name="Haurand1985”>[www.ncbi.nlm.nih.gov/pubmed/2999104 Haurand M. “Isolation and characterization of thromboxane synthase from human platelets as a cytochrome P-450 enzyme” J Biol Chem. 1985, 260 (5), 15059-15067]</ref> | ||
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Revision as of 13:53, 28 May 2016
Contents
Reaction
Chemical equation
Rate equation
Parameters
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 4.00E-03 | 
|
Human | Expression Vector: Human Monocyte
Enzyme: Platelet Free Human Monocyte pH: 7.4 Temperature: 37 |
[1] |
| 1.00E-02 |
|
Human | Expression Vector: Human mirosome
Enzyme: Human Thromboxane Synthase pH:7.4 Temperature: |
[2] |
| 2.20E-02 |
|
Human | Expression Vector:Human platelet
Enzyme: Human thromboxane synthase pH: 7.4 Temperature: 37 |
[3] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 2687 | per minute | Human | Expression Vector: Human Platelets
Enzyme: TXAS pH: 7.4 Temperature:30 |
[4] |
| 1628 | per minute | Human | Expression Vector:Platelet Microsomes
Enzyme: TXAS pH:7.4 Temperature:37 |
[5] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 2.04E-05 | mM | Human | 1.02e-8 mmol divided by volume (0.0005 l). | [4] |
| 2.21E-07 | mM | Human | Calculated by Mw (58800), Protein concentration (13 mg/ml).
Human platlets. |
[5] |
References
- ↑ M. Orlandi, G. Bartolini, Thromboxane A2 synthase activity in platelet free human monocytes. Biochim Biophys Acta. 1994 Dec 8;1215(3):285-90.
- ↑ R. Nusing, S. Schneider-Voss and V. Ullrich, Immunoaffinity purification of human thromboxane synthase. Arch Biochem Biophys. 1990 Aug 1;280(2):325-30.
- ↑ Hecker M. On the mechanism of prostacyclin and thromboxane A2 biosynthesis.J Biol Chem. 1989 Jan 5;264(1):141-50.
- ↑ 4.0 4.1 [www.ncbi.nlm.nih.gov/pubmed/3579292 Hecker M. “Products, kinetics, and substrate specificity of homogeneous thromboxane synthase from human platelets: Development of a novel enzyme assay ” Arch. Biochem. Biophys. 1987, 254, 124-135]
- ↑ 5.0 5.1 [www.ncbi.nlm.nih.gov/pubmed/2999104 Haurand M. “Isolation and characterization of thromboxane synthase from human platelets as a cytochrome P-450 enzyme” J Biol Chem. 1985, 260 (5), 15059-15067]
