Difference between revisions of "Transformation of PGH2 to TXA2"
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|<ref name="Haurand1985”>[www.ncbi.nlm.nih.gov/pubmed/2999104 Haurand M. “Isolation and characterization of thromboxane synthase from human platelets as a cytochrome P-450 enzyme” J Biol Chem. 1985, 260 (5), 15059-15067]</ref> | |<ref name="Haurand1985”>[www.ncbi.nlm.nih.gov/pubmed/2999104 Haurand M. “Isolation and characterization of thromboxane synthase from human platelets as a cytochrome P-450 enzyme” J Biol Chem. 1985, 260 (5), 15059-15067]</ref> | ||
|- | |- | ||
| + | |} | ||
| + | |||
| + | {|class="wikitable sortable" | ||
| + | |+ style="text-align: left;" | Enzyme Concentrations | ||
| + | |- | ||
| + | ! Value | ||
| + | ! Units | ||
| + | ! Species | ||
| + | ! Notes | ||
| + | ! Reference | ||
| + | |- | ||
| + | |2.04E-05 | ||
| + | |mM | ||
| + | |Human | ||
| + | |1.02e-8 mmol divided by volume (0.0005 l). | ||
| + | |<ref name="Hecker1987”>[www.ncbi.nlm.nih.gov/pubmed/3579292 Hecker M. “Products, kinetics, and substrate specificity of homogeneous thromboxane synthase from human platelets: Development of a novel enzyme assay ” Arch. Biochem. Biophys. 1987, 254, 124-135]</ref> | ||
| + | |- | ||
| + | |2.21E-07 | ||
| + | |mM | ||
| + | |Human | ||
| + | |Calculated by Mw (58800), Protein concentration (13 mg/ml). | ||
| + | Human platlets. | ||
| + | ||<ref name="Haurand1985”>[www.ncbi.nlm.nih.gov/pubmed/2999104 Haurand M. “Isolation and characterization of thromboxane synthase from human platelets as a cytochrome P-450 enzyme” J Biol Chem. 1985, 260 (5), 15059-15067]</ref> | ||
|} | |} | ||
Revision as of 16:28, 26 April 2016
Contents
Reaction
Chemical equation
Rate equation
Parameters
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 4.00E-03 | 
|
Human | Platelet Free Human Monocyte | [1] |
| 1.00E-02 |
|
Human | Human Thromboxane Synthase | [2] |
| 2.20E-02 |
|
Human | Wild-Type TXAS | [3] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 2687 | per minute | Human | Platelets | [4] |
| 1628 | per minute | Human | Platelet Microsomes | [5] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 2.04E-05 | mM | Human | 1.02e-8 mmol divided by volume (0.0005 l). | [4] |
| 2.21E-07 | mM | Human | Calculated by Mw (58800), Protein concentration (13 mg/ml).
Human platlets. |
[5] |
References
- ↑ M. Orlandi, G. Bartolini, Thromboxane A2 synthase activity in platelet free human monocytes. Biochim Biophys Acta. 1994 Dec 8;1215(3):285-90.
- ↑ R. Nusing, S. Schneider-Voss and V. Ullrich, Immunoaffinity purification of human thromboxane synthase. Arch Biochem Biophys. 1990 Aug 1;280(2):325-30.
- ↑ Hecker M. On the mechanism of prostacyclin and thromboxane A2 biosynthesis.J Biol Chem. 1989 Jan 5;264(1):141-50.
- ↑ 4.0 4.1 [www.ncbi.nlm.nih.gov/pubmed/3579292 Hecker M. “Products, kinetics, and substrate specificity of homogeneous thromboxane synthase from human platelets: Development of a novel enzyme assay ” Arch. Biochem. Biophys. 1987, 254, 124-135]
- ↑ 5.0 5.1 [www.ncbi.nlm.nih.gov/pubmed/2999104 Haurand M. “Isolation and characterization of thromboxane synthase from human platelets as a cytochrome P-450 enzyme” J Biol Chem. 1985, 260 (5), 15059-15067]
