Difference between revisions of "Transformation of PGH2 to PGI2"

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== Parameters ==
 
== Parameters ==
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=== K<sub>ms</sub> ===
  
 
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|+  style="text-align: left;" | Michaelis-Menten Constants
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{| class="wikitable"
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|+  style="text-align: left;" | Description of the PGIS Kms distribution
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! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
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|-
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| 1.24E-02 || 2.46E+00 || -4.22E+00 || 4.20E-01
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|}
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[[Image:17.jpg|none|thumb|500px|The estimated probability distribution for PGIS Kms. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.  ]]
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=== K<sub>mp</sub> ===
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===k<sub>cat</sub> ===
  
 
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|+  style="text-align: left;" | Enzyme Turnover Numbers
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! Value
 
! Value
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| <ref name="Hara1994"> [www.ncbi.nlm.nih.gov/pubmed/8051072 Hara S. , ''Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells.'' J Biol Chem. 1994 Aug 5;269(31):19897-903.]</ref>  
 
| <ref name="Hara1994"> [www.ncbi.nlm.nih.gov/pubmed/8051072 Hara S. , ''Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells.'' J Biol Chem. 1994 Aug 5;269(31):19897-903.]</ref>  
 
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=== Enzyme concentration ===
  
 
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|+  style="text-align: left;" | PGIS Abundance
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=== K<sub>eq</sub> ===
 
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|+  style="text-align: left;" | Gibbs Free Energy Change
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! Value
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! Gibbs Free Energy Change
 
! Units
 
! Units
 
! Species
 
! Species

Revision as of 08:06, 15 May 2019

Return to overview

PGH2 is metabolised into the prostacyclin PGI2, by PGIS. This species has been associated with influencing the permeability of vascular compartment (Murata, Ushikubi et al. 1997). Similarly to the TX species, prostacyclins are only found in low concentration and are associated with infiltrating cells from the vascular compartment (Sugimoto, Arai et al. 2006).

Reaction

R5 PGH2 - PGI2.jpg

Chemical equation

PGH2 \rightleftharpoons PGI2

Rate equation

R05.PNG

Parameters

Kms

Literature values
Value Units Species Notes Reference
1.33E-02 ± 1.40E-03 mM Human Expression Vector: Bovine Endothelial and Aorta Cells

Enzyme: Human PGIS pH:7.4 Temperature: 23

[1]
9.00E-03 ± 5.00E-03 mM Bovine Expression Vector: Bovine Endothelial and Aorta Cells

Enzyme: Bovine PGIS pH:7.4 Temperature: 24

[2]
Description of the PGIS Kms distribution
Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
1.24E-02 2.46E+00 -4.22E+00 4.20E-01
The estimated probability distribution for PGIS Kms. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

Kmp

kcat

Literature values
Value Units Species Notes Reference
147 ± 45 per minute Cattle Expression Vector: E. Coli

Enzyme: Bovine PGIS pH:7.4 Temperature: 24

[2]



Enzyme concentration

Literature values
Value Units Species Notes Reference
412 ppm Human Expression Vector: Urinary bladder

Enzyme: PGIS pH: 7.5 Temperature: 37 °C

[3]
206 ppm Human Expression Vector: Lung

Enzyme: PGIS pH: 7.5 Temperature: 37 °C

[3]
60.1 ppm Human Expression Vector: Esophagus

Enzyme: PGIS pH: 7.5 Temperature: 37 °C

[3]
9.93 ppm Human Expression Vector: Oral Cavity

Enzyme: PGIS pH: 7.5 Temperature: 37 °C

[4]


Keq

Literature values
Gibbs Free Energy Change Units Species Notes Reference
0.04 kcal/mol Not stated Estimated

Enzyme: Transacylase Substrate: Product: pH: 7.3 ionic strength: 0.25

[5]

References

  1. ↑ H. C. Yeh, P. Y. Hsu, Characterization of heme environment and mechanism of peroxide bond cleavage in human prostacyclin synthase. Biochim Biophys Acta. 2005 Dec 30;1738(1-3):121-32.
  2. ↑ 2.0 2.1 Hara S. , Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells. J Biol Chem. 1994 Aug 5;269(31):19897-903. Cite error: Invalid <ref> tag; name "Hara1994" defined multiple times with different content
  3. ↑ 3.0 3.1 3.2 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  4. ↑ M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
  5. ↑ Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471

Related Reactions

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