Difference between revisions of "Transformation of PGH2 to PGI2"

Revision as of 15:24, 18 August 2016

FA \rightleftharpoons AA

Michaelis-Menten Constants
Value	Units	Species	Notes	Reference
1.33E-02 ± 1.40E-03	$mM$	Human	Expression Vector: Bovine Endothelial and Aorta Cells Enzyme: Human PGIS pH:7.4 Temperature: 23	^[1]
9.00E-03 ± 5.00E-03	$mM$	Bovine	Expression Vector: Bovine Endothelial and Aorta Cells Enzyme: Bovine PGIS pH:7.4 Temperature: 24	^[2]

Enzyme Turnover Numbers
Value	Units	Species	Notes	Reference
147 ± 45	per minute	Cattle	Expression Vector: E. Coli Enzyme: Bovine PGIS pH:7.4 Temperature: 24	^[2]

PGIS Abundance
Value	Units	Species	Notes	Reference
412	$ppm$	Human	Expression Vector: Urinary bladder Enzyme: PGIS pH: 7.5 Temperature: 37 °C	^[3]
206	$ppm$	Human	Expression Vector: Lung Enzyme: PGIS pH: 7.5 Temperature: 37 °C	^[3]
60.1	$ppm$	Human	Expression Vector: Esophagus Enzyme: PGIS pH: 7.5 Temperature: 37 °C	^[3]
9.93	$ppm$	Human	Expression Vector: Oral Cavity Enzyme: PGIS pH: 7.5 Temperature: 37 °C	^[4]

Gibbs Free Energy Change
Value	Units	Species	Notes	Reference
0.04	kcal/mol	Not stated	Estimated Enzyme: pgis Substrate: Arachidonate Product: PGI2 pH: 7.3 ionic strength: 0.25	^[5]

@@ Line 105: / Line 105: @@
 |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 |-
+|}
+{|class="wikitable sortable"
+|+  style="text-align: left;" | Gibbs Free Energy Change
+|-
+! Value
+! Units
+! Species
+! Notes
+! Reference
+|-
+|0.04
+|kcal/mol
+|Not stated
+|Estimated
+Enzyme: pgis
+Substrate: Arachidonate
+Product: PGI2
+pH: 7.3
+ionic strength: 0.25
+|<ref name="MetaCyc”>[http://metacyc.org/META/NEW-IMAGE?type=REACTION&object=PROSTAGLANDIN-I-SYNTHASE-RXN Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471]</ref>
 |}