Difference between revisions of "Transformation of PGH2 to PGI2"

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Temperature: 24
 
Temperature: 24
 
| <ref name="Hara1994"> [www.ncbi.nlm.nih.gov/pubmed/8051072 Hara S. , ''Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells.'' J Biol Chem. 1994 Aug 5;269(31):19897-903.]</ref>  
 
| <ref name="Hara1994"> [www.ncbi.nlm.nih.gov/pubmed/8051072 Hara S. , ''Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells.'' J Biol Chem. 1994 Aug 5;269(31):19897-903.]</ref>  
 +
|}
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{|class="wikitable sortable"
 +
|+  style="text-align: left;" | PGIS Abundance
 +
|-
 +
! Value
 +
! Units
 +
! Species
 +
! Notes
 +
! Reference
 +
|-
 +
|412
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Urinary bladder
 +
Enzyme: PGIS
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 +
|-
 +
|206
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Lung
 +
Enzyme: PGIS
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 +
|-
 +
|60.1
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Esophagus
 +
Enzyme: PGIS
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 +
|-
 +
|9.93
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Oral Cavity
 +
Enzyme: PGIS
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 +
|-
 
|}
 
|}
  

Revision as of 11:49, 9 August 2016

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Reaction

R5 PGH2 - PGI2.jpg

Chemical equation

FA \rightleftharpoons AA

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
1.33E-02 ± 1.40E-03 mM Human Expression Vector: Bovine Endothelial and Aorta Cells

Enzyme: Human PGIS pH:7.4 Temperature: 23

[1]
9.00E-03 ± 5.00E-03 mM Bovine Expression Vector: Bovine Endothelial and Aorta Cells

Enzyme: Bovine PGIS pH:7.4 Temperature: 24

[2]
Enzyme Turnover Numbers
Value Units Species Notes Reference
147 ± 45 per minute Cattle Expression Vector: E. Coli

Enzyme: Bovine PGIS pH:7.4 Temperature: 24

[2]
PGIS Abundance
Value Units Species Notes Reference
412 ppm Human Expression Vector: Urinary bladder

Enzyme: PGIS pH: 7.5 Temperature: 37 °C

[3]
206 ppm Human Expression Vector: Lung

Enzyme: PGIS pH: 7.5 Temperature: 37 °C

[3]
60.1 ppm Human Expression Vector: Esophagus

Enzyme: PGIS pH: 7.5 Temperature: 37 °C

[3]
9.93 ppm Human Expression Vector: Oral Cavity

Enzyme: PGIS pH: 7.5 Temperature: 37 °C

[4]

References

  1. ↑ H. C. Yeh, P. Y. Hsu, Characterization of heme environment and mechanism of peroxide bond cleavage in human prostacyclin synthase. Biochim Biophys Acta. 2005 Dec 30;1738(1-3):121-32.
  2. ↑ 2.0 2.1 Hara S. , Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells. J Biol Chem. 1994 Aug 5;269(31):19897-903. Cite error: Invalid <ref> tag; name "Hara1994" defined multiple times with different content
  3. ↑ 3.0 3.1 3.2 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  4. ↑ M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587

Related Reactions

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