Difference between revisions of "Transformation of PGH2 to PGI2"
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| Line 24: | Line 24: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | |Expression Vector: | + | |Expression Vector: Bovine Endothelial and Aorta Cells |
Enzyme: Human PGIS | Enzyme: Human PGIS | ||
pH:7.4 | pH:7.4 | ||
| Line 33: | Line 33: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Bovine | |Bovine | ||
| − | |Expression Vector: | + | |Expression Vector: Bovine Endothelial and Aorta Cells |
Enzyme: Bovine PGIS | Enzyme: Bovine PGIS | ||
pH:7.4 | pH:7.4 | ||
Revision as of 12:36, 28 May 2016
Contents
Reaction
Chemical equation
Rate equation
Parameters
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 1.33E-02 ± 1.40E-03 | 
|
Human | Expression Vector: Bovine Endothelial and Aorta Cells
Enzyme: Human PGIS pH:7.4 Temperature: 23 |
[1] |
| 9.00E-03 ± 5.00E-03 |
|
Bovine | Expression Vector: Bovine Endothelial and Aorta Cells
Enzyme: Bovine PGIS pH:7.4 Temperature: 24 |
[2] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 147 ± 45 | per minute | Cattle | Expression Vector: E. Coli
Enzyme: Bovine PGIS pH:7.4 Temperature: 24 |
[2] |
References
- ↑ H. C. Yeh, P. Y. Hsu, Characterization of heme environment and mechanism of peroxide bond cleavage in human prostacyclin synthase. Biochim Biophys Acta. 2005 Dec 30;1738(1-3):121-32.
- ↑ 2.0 2.1 Hara S. , Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells. J Biol Chem. 1994 Aug 5;269(31):19897-903. Cite error: Invalid
<ref>tag; name "Hara1994" defined multiple times with different content
