Difference between revisions of "Transformation of PGH2 to PGI2"

Revision as of 12:35, 28 May 2016

FA \rightleftharpoons AA

Michaelis-Menten Constants
Value	Units	Species	Notes	Reference
1.33E-02 ± 1.40E-03	$mM$	Human	Expression Vector: E. Coli Enzyme: Human PGIS pH:7.4 Temperature: 23	^[1]
9.00E-03 ± 5.00E-03	$mM$	Bovine	Expression Vector: E. Coli Enzyme: Bovine PGIS pH:7.4 Temperature: 24	^[2]

Enzyme Turnover Numbers
Value	Units	Species	Notes	Reference
147 ± 45	per minute	Cattle	Expression Vector: E. Coli Enzyme: Bovine PGIS pH:7.4 Temperature: 24	^[2]

@@ Line 33: / Line 33: @@
 |<math> mM </math>
 |Bovine
-|Bovine Endothelial Cells
+|Expression Vector: E. Coli
+Enzyme: Bovine PGIS
+pH:7.4
+Temperature: 24
 |<ref name="Hara1994"> [http://www.ncbi.nlm.nih.gov/pubmed/8051072 Hara S. , ''Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells.'' J Biol Chem. 1994 Aug 5;269(31):19897-903.]</ref>
 |-
@@ Line 50: / Line 53: @@
 | per minute
 | Cattle
-| Bovine aorta microsomes
+|Expression Vector: E. Coli
+Enzyme: Bovine PGIS
+pH:7.4
+Temperature: 24
 | <ref name="Hara1994"> [www.ncbi.nlm.nih.gov/pubmed/8051072 Hara S. , ''Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells.'' J Biol Chem. 1994 Aug 5;269(31):19897-903.]</ref>
 |}