Difference between revisions of "Transformation of PGH2 to PGI2"
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|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | |Human PGIS | + | |Expression Vector: E. Coli |
| + | Enzyme: Human PGIS | ||
| + | pH:7.4 | ||
| + | Temperature: 23 | ||
|<ref name="Yeh2005"> [http://www.ncbi.nlm.nih.gov/pubmed/16406803 H. C. Yeh, P. Y. Hsu, Characterization of heme environment and mechanism of peroxide bond cleavage in human prostacyclin synthase.'' Biochim Biophys Acta. 2005 Dec 30;1738(1-3):121-32.]</ref> | |<ref name="Yeh2005"> [http://www.ncbi.nlm.nih.gov/pubmed/16406803 H. C. Yeh, P. Y. Hsu, Characterization of heme environment and mechanism of peroxide bond cleavage in human prostacyclin synthase.'' Biochim Biophys Acta. 2005 Dec 30;1738(1-3):121-32.]</ref> | ||
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Revision as of 12:19, 28 May 2016
Contents
Reaction
Chemical equation
Rate equation
Parameters
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 1.33E-02 ± 1.40E-03 | 
|
Human | Expression Vector: E. Coli
Enzyme: Human PGIS pH:7.4 Temperature: 23 |
[1] |
| 9.00E-03 ± 5.00E-03 |
|
Bovine | Bovine Endothelial Cells | [2] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 147 ± 45 | per minute | Cattle | Bovine aorta microsomes | [2] |
References
- ↑ H. C. Yeh, P. Y. Hsu, Characterization of heme environment and mechanism of peroxide bond cleavage in human prostacyclin synthase. Biochim Biophys Acta. 2005 Dec 30;1738(1-3):121-32.
- ↑ 2.0 2.1 Hara S. , Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells. J Biol Chem. 1994 Aug 5;269(31):19897-903. Cite error: Invalid
<ref>tag; name "Hara1994" defined multiple times with different content
