Difference between revisions of "Transformation of PGF2a to exPGF2a by PGT"

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== Transport Parameters ==
 
== Transport Parameters ==
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===K<sub>ms</sub>===
 
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|<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
 
|<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
 
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{| class="wikitable"
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! Mode (mM) !! Confidence Interval  !! Location parameter (µ) !! Scale parameter (σ)
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| 1.10E-03 || 9.42E+01 || -4.67E+00 || 1.45E+00
 
|}
 
|}
  
  
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===k<sub>cat</sub>===
 
No data available on the turnover of PGT/ an organic molecule transporter therefore based upon estimates made by http://book.bionumbers.org/what-are-the-rates-of-membrane-transporters/
 
No data available on the turnover of PGT/ an organic molecule transporter therefore based upon estimates made by http://book.bionumbers.org/what-are-the-rates-of-membrane-transporters/
 
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{| class="wikitable"
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! Mode (min-1) !! Confidence Interval  !! Location parameter (µ) !! Scale parameter (σ)
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| 1.33E+00 || 7.30E+00 || 9.20E-01 || 8.00E-01
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=== Transporter concentration ===
 
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{| class="wikitable"
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! Mode (mM) !! Confidence Interval  !! Location parameter (µ) !! Scale parameter (σ)
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|-
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| 1.73E+01 || 4.72E+00 || 3.90E+00 || 1.02E+00
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== References ==
 
== References ==
 
<references/>
 
<references/>

Revision as of 15:15, 16 May 2019

Return to overview

Prostaglandin Transporter


Rate equation

R101.PNG

Transport Parameters

Kms

Literature values
Value Units Species Notes Reference
0.0054 mM Human Eye Vector: HEK-OATP2A1 or HEK-OATP2B1 and the respective vector controls

Substrate: Latanoprost, a prostaglandin F2a analogue pH: 7.5 Temp: 37 °C

[1]
0.000331 ± 0.000131 mM Transfected Human embryonic kidney cells (HEK293) Substrate: PGE2

pH: unknown Temp: 37 °C

[2]
0.007202 ± 0.000595 mM Transfected Human embryonic kidney cells (HEK293) Substrate: PGE3

pH: unknown Temp: 37 °C

[2]
0.000376 ± 3.4e-5 mM PGT-expressing Madin-Darby Canine Kidney Epithelial Cells (MDCK) cells Substrate: PGH2

pH: unknown Temp: unknown

[3]
9.40E-05 mM Human Substrate: PGE2

Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27

[4]
0.000423 mM Human Substrate: TXB2

Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27

[4]
0.007569 mM Human Substrate: 6-Keto-PGF1a

Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27

[4]
4.87E-05 mM Human Substrate: PGE2

Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27

[5]
5.02E-05 mM Human Substrate: PGF2a

Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27

[5]
0.005 mM Human Substrate: 15-Keto-PGE2

Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27

[5]
0.005 mM Human Substrate: 13,14-Dihydo-15-Keto-PGE2

Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27

[5]
3.82E-05 mM Human Substrate: PGH2

Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27

[5]
Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
1.10E-03 9.42E+01 -4.67E+00 1.45E+00


kcat

No data available on the turnover of PGT/ an organic molecule transporter therefore based upon estimates made by http://book.bionumbers.org/what-are-the-rates-of-membrane-transporters/

Turnover Number
Value Units Transporter Notes Reference
3.5 min-1 Glucose transporter ptsI Organism: Bacteria Escherichia coli

Back of the envelope calculation by BioNumbers: http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=102931

[6]
0.33 - 0.83 min-1 Rate of transport of H+/Lactose transporter Organism: Bacteria Escherichia coli

Back of the envelop calculation on bionumbers http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=103159

[7]
3.28 min-1 Catalytic Rate of transporter HXT7 Organism: Budding yeast Saccharomyces cerevisiae

Back of the envelope calculation http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=101737

[8]
Mode (min-1) Confidence Interval Location parameter (µ) Scale parameter (σ)
1.33E+00 7.30E+00 9.20E-01 8.00E-01


Transporter concentration

Abundance
Value Units Species Notes Reference
125 ppm Human Vector:Lung

Enzyme: SLCO2A1 pH: 7.5 Temperature: 37 °C

[9]
17.4 ppm Human Expression Vector: Esophagus

Enzyme: SLCO2A1 pH: 7.5 Temperature: 37 °C

[10]
2.82 ppm Human Vector:Gut

Enzyme: SLCO2A1 pH: 7.5 Temperature: 37 °C

[9]
Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
1.73E+01 4.72E+00 3.90E+00 1.02E+00

References

  1. ↑ M. Kraft The prostaglandin transporter OATP2A1 is expressed in human ocular tissues and transports the antiglaucoma prostanoid latanoprost. Invest Ophthalmol Vis Sci. 2010 May;51(5):2504-11. doi: 10.1167/iovs.09-4290. Epub 2009 Dec 17.
  2. ↑ 2.0 2.1 Gose T. Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3. Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.
  3. ↑ Chi Y. The prostaglandin transporter PGT transports PGH2 Biochemical and Biophysical Research Communications, Volume 395, Issue 2, 30 April 2010, Pages 168–172
  4. ↑ 4.0 4.1 4.2 [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. Identification and Characterization of a Prostaglandin Transporter Science 12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]
  5. ↑ 5.0 5.1 5.2 5.3 5.4 Shigekazu I. Structural Determinants of Substrates for the Prostaglandin Transporter PGT Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736
  6. ↑ Waygood EB Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties.Can J Biochem. 1980 Jan;58(1):40-8.
  7. ↑ Naftalin R. J. ELactose permease H+-lactose symporter: mechanical switch or Brownian ratchet?Biophys J. 2007 May 15;92(10):3474-91. Epub 2007 Feb 26.
  8. ↑ Ye L. Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiaeYeast. 2001 Sep 30;18(13):1257-67.
  9. ↑ 9.0 9.1 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  10. ↑ M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
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