Transformation of PGE2 to 15-Keto-PGE2

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15-hydroxyprostaglandin dehydrogenase, also known as (15-PGDH) metabolises PG into the 15-Keto variant via a ketone reduction. The enzyme is constituently expressed in the skin (Finhelm1982) and within the eicosanoid network it is reported as metabolising PGE2 and PGF2a.

It should be noted that Judson et al found that 15-PGDH expression of the enzyme decreases in response to UVR (Judson2010).

Reaction

Catalysed by 15-PGDH

Chemical equation

$PGE2 \rightleftharpoons 15-Keto-PGE2$

15-PGDH Parameters

Michaelis-Menten Constants

Value	Units	Species	Notes	Reference
0.008	$mM$	Rat Skin	Method: pH:7.4 Temperature: 37'C Substrate: PGE2 + NAD+
0.0075	$mM$	Rat Skin	Method: pH:7.4 Temperature: 37'C Substrate: PGE2 + NADP+
0.024	$mM$	Rat Skin	Method: pH:7.4 Temperature: 37'C Substrate: PGF2a + NAD+
0.023	$mM$	Rat Skin	Method: pH:7.4 Temperature: 37'C Substrate: PGF2a + NADP+
0.0039	$mM$	Purified Human 15-PGDH	Method: In vitro Expression Vector: E. coli pH:7.5 Temperature: 37'C Substrate: PGE2
0.0099	$mM$	Purified Rat 15-PGDH	Method: In vitro Expression Vector: E. coli pH:7.5 Temperature: 37'C Substrate: PGE2
0.0055 ± 0.0006	$mM$	Human	Method:In vitro Expression Vector: E. Coli pH:8 Temperature: 25 Substrate: PGE2 + NAD+

Enzyme Turnover Numbers

Value	Units	Species	Notes	Reference
816 ± 18	$per minute$	Human	Method: In vitro Expression Vector: E. Coli pH:8 Temperature: 25 Substrate: PGE2 + NAD+
366 ± 12 - 846 ± 12	$per minute$	Human	Method: In vitro Expression Vector: E. Coli pH:8 Temperature: 25 Substrate: PGE2 + NAD+ + Inhibitor

cPLA2 Abundance

Value	Units	Species	Notes	Reference
	$ppm$

Gibbs Free Energy Change

Value	Units	Species	Notes	Reference