Transformation of PGE2 to 15-Keto-PGE2

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15-hydroxyprostaglandin dehydrogenase, also known as (15-PGDH) metabolises PG into the 15-Keto variant via the oxidation of 15(S)-hydroxyl group. The enzyme is constituently expressed in the skin (Finhelm1982) and within the eicosanoid network it is reported as metabolising PGE2 and PGF2a.

It should be noted that Judson et al found that 15-PGDH expression of the enzyme decreases in response to UVR (Judson2010).

Reaction

Chemical equation

$PGE2 \rightleftharpoons 15-Keto-PGE2$

Rate equation

Enzyme Parameters

K_ms

Literature values

Value	Units	Species	Notes	Reference
0.008	$mM$	Rat Skin	Method: pH:7.4 Temperature: 37'C Substrate: PGE2 + NAD+
0.0075	$mM$	Rat Skin	Method: pH:7.4 Temperature: 37'C Substrate: PGE2 + NADP+
0.024	$mM$	Rat Skin	Method: pH:7.4 Temperature: 37'C Substrate: PGF2a + NAD+
0.023	$mM$	Rat Skin	Method: pH:7.4 Temperature: 37'C Substrate: PGF2a + NADP+
0.0039	$mM$	Purified Human 15-PGDH	Method: In vitro Expression Vector: E. coli pH:7.5 Temperature: 37'C Substrate: PGE2
0.0099	$mM$	Purified Rat 15-PGDH	Method: In vitro Expression Vector: E. coli pH:7.5 Temperature: 37'C Substrate: PGE2
0.0055 ± 0.0006	$mM$	Human	Method:In vitro Expression Vector: E. Coli pH:8 Temperature: 25 Substrate: PGE2 + NAD+

Description of the 15-PGDH Kms distribution

Mode (mM)	Confidence Interval	Location parameter (μ)	Scale parameter (σ)
7.64E-03	5.05E+00	-4.41E+00	6.80E-01

The estimated probability distribution for 15-PGDH Kms. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

K_mp

Description of the 15-PGDH Kmp distribution

Mode (mM)	Location parameter (μ)	Scale parameter (σ)
7.70E-03	-4.41E+00	6.72E-01

The estimated probability distribution for 15-PGDH Kmp. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

k_cat

Literature values

Value	Units	Species	Notes	Reference
816 ± 18	$per minute$	Human	Method: In vitro Expression Vector: E. Coli pH:8 Temperature: 25 Substrate: PGE2 + NAD+
366 ± 12 - 846 ± 12	$per minute$	Human	Method: In vitro Expression Vector: E. Coli pH:8 Temperature: 25 Substrate: PGE2 + NAD+ + Inhibitor

Description of the 15-PGDH kcat distribution

Mode (min-1)	Confidence Interval	Location parameter (μ)	Scale parameter (σ)
8.12E+02	5.38E+00	7.19E+00	7.01E-01

The estimated probability distribution for 15-PGDH kcat. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

Enzyme concentration

Literature values

Value	Units	Species	Notes	Reference
69.8	$ppm$	Human	Expression Vector: Esophagus Enzyme: 15-PGDH pH: 7.5 Temperature: 37 °C
22.1	$ppm$	Human	Expression Vector: Heart Enzyme: 15-PGDH pH: 7.5 Temperature: 37 °C
11.2	$ppm$	Human	Expression Vector: Esophagus Enzyme: 15-PGDH pH: 7.5 Temperature: 37 °C
7.68	$ppm$	Human	Expression Vector: Skin Enzyme: 15-PGDH pH: 7.5 Temperature: 37 °C	Paxdb - Unknown

Description of the 15-PGDH concentration distribution

Mode (mM)	Confidence Interval	Location parameter (μ)	Scale parameter (σ)
1.12E+01	2.32E+00	2.87E+00	6.80E-01

The estimated probability distribution for 15-PGDH concentration. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

K_eq

Gibbs Free Energy Change

Value	Units	Species	Notes	Reference
(-0.46818542)	kcal/mol	Calculated	Estimated Enzyme: 15-PGDH Substrate: PGE2 Product: 15-dehydro-PGE2 pH: 7.3 ionic strength: 0.25

Description of the 15-PGDH Keq distribution

Mode	Confidence Interval	Location parameter (μ)	Scale parameter (σ)
2.21E+00	1.00E+01	1.58E+00	8.91E-01

The estimated probability distribution for 15-PGDH Keq. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.