Difference between revisions of "Transformation of PGE2 to 15-Keto-PGE2"
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− | |+ style="text-align: left;" | | + | |+ style="text-align: left;" | 15-PGDH Abundance |
|- | |- | ||
! Value | ! Value | ||
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! Reference | ! Reference | ||
|- | |- | ||
− | | | + | |69.8 |
|<math> ppm </math> | |<math> ppm </math> | ||
− | | | + | |Human |
− | | | + | |Expression Vector: Esophagus |
− | | | + | Enzyme: 15-PGDH |
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref> | ||
|- | |- | ||
+ | |22.1 | ||
+ | |<math> ppm </math> | ||
+ | |Human | ||
+ | |Expression Vector: Heart | ||
+ | Enzyme: 15-PGDH | ||
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref> | ||
+ | |- | ||
+ | |11.2 | ||
+ | |<math> ppm </math> | ||
+ | |Human | ||
+ | |Expression Vector: Esophagus | ||
+ | Enzyme: 15-PGDH | ||
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref> | ||
+ | |- | ||
+ | |7.68 | ||
+ | |<math> ppm </math> | ||
+ | |Human | ||
+ | |Expression Vector: Skin | ||
+ | Enzyme: 15-PGDH | ||
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |Paxdb - Unknown | ||
|} | |} | ||
Revision as of 16:44, 28 September 2016
15-hydroxyprostaglandin dehydrogenase, also known as (15-PGDH) metabolises PG into the 15-Keto variant via a ketone reduction. The enzyme is constituently expressed in the skin (Finhelm1982) and within the eicosanoid network it is reported as metabolising PGE2 and PGF2a.
It should be noted that Judson et al found that 15-PGDH expression of the enzyme decreases in response to UVR (Judson2010).
Reaction
Catalysed by 15-PGDH
Chemical equation
Rate equation
15-PGDH Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
0.008 | Rat Skin | Method:
pH:7.4 Temperature: 37'C Substrate: PGE2 + NAD+ |
[1] | |
0.0075 | Rat Skin | Method:
pH:7.4 Temperature: 37'C Substrate: PGE2 + NADP+ |
[1] | |
0.024 | Rat Skin | Method:
pH:7.4 Temperature: 37'C Substrate: PGF2a + NAD+ |
[1] | |
0.023 | Rat Skin | Method:
pH:7.4 Temperature: 37'C Substrate: PGF2a + NADP+ |
[1] | |
0.0039 | Purified Human 15-PGDH | Method: In vitro
Expression Vector: E. coli pH:7.5 Temperature: 37'C Substrate: PGE2 |
[2] | |
0.0099 | Purified Rat 15-PGDH | Method: In vitro
Expression Vector: E. coli pH:7.5 Temperature: 37'C Substrate: PGE2 |
[2] | |
0.0055 ± 0.0006 | Human | Method:In vitro
Expression Vector: E. Coli pH:8 Temperature: 25 Substrate: PGE2 + NAD+ |
[3] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
816 ± 18 | Human | Method: In vitro
Expression Vector: E. Coli pH:8 Temperature: 25 Substrate: PGE2 + NAD+ |
[3] | |
366 ± 12 - 846 ± 12 | Human | Method: In vitro
Expression Vector: E. Coli pH:8 Temperature: 25 Substrate: PGE2 + NAD+ + Inhibitor |
[3] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
69.8 | Human | Expression Vector: Esophagus
Enzyme: 15-PGDH pH: 7.5 Temperature: 37 °C |
[4] | |
22.1 | Human | Expression Vector: Heart
Enzyme: 15-PGDH pH: 7.5 Temperature: 37 °C |
[5] | |
11.2 | Human | Expression Vector: Esophagus
Enzyme: 15-PGDH pH: 7.5 Temperature: 37 °C |
[5] | |
7.68 | Human | Expression Vector: Skin
Enzyme: 15-PGDH pH: 7.5 Temperature: 37 °C |
Paxdb - Unknown |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
References
- ↑ 1.0 1.1 1.2 1.3 N. Fincham, Novel prostaglandin dehydrogenase in rat skin. Biochem J. 1983 Apr 15;212(1):129-34.
- ↑ 2.0 2.1 Zhou H., C-Terminal region of human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase is involved in the interaction with prostaglandin substrates. Eur J Biochem. 2001 Jun;268(12):3368-74.
- ↑ 3.0 3.1 3.2 F. Niesen,, High-Affinity Inhibitors of Human NAD+-Dependent 15-Hydroxyprostaglandin Dehydrogenase: Mechanisms of Inhibition and Structure-Activity Relationships PLoS One. 2010 Nov 2;5(11):e13719.
- ↑ M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
- ↑ 5.0 5.1 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581