Difference between revisions of "Transformation of LTA4 to LTB4"
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===Enzyme concentration=== | ===Enzyme concentration=== | ||
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+ | To convert the enzyme concentration from ppm to mM, the following [[Common equations#Enzyme concentration (mM)|equation]] was used. | ||
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{| class="wikitable" | {| class="wikitable" | ||
|+ style="text-align: left;" | Description of the LTA4H concentration distribution | |+ style="text-align: left;" | Description of the LTA4H concentration distribution | ||
− | ! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ) | + | ! Mode (ppm) !! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ) |
|- | |- | ||
− | | 4.88E+02 || 1.25E+00 || 6.24E+00 || 2.19E-01 | + | | 4.88E+02 ||2.70E-03|| 1.25E+00 || 6.24E+00 || 2.19E-01 |
|} | |} | ||
[[Image:lta4h.jpg|none|thumb|500px|The estimated probability distribution for LTA4H concentration. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale. ]] | [[Image:lta4h.jpg|none|thumb|500px|The estimated probability distribution for LTA4H concentration. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale. ]] | ||
− | |||
===K<sub>eq</sub>=== | ===K<sub>eq</sub>=== |
Revision as of 10:46, 30 May 2019
Contents
Reaction
Chemical equation
Rate equation
Parameters
Kms
Value | Units | Species | Notes | Weight | Reference |
---|---|---|---|---|---|
5.80E-03 | mM | Human | Expression Vector: E. Coli
Enzyme: Wild Type Leukotriene A4 hydrolase: pH:8 Temperature: 20 (Room Temperature) |
128 | [1] |
2.70E-03 | mM | Frog | Expression Vector: oocytes
Enzyme: leukotriene A4 hydrolase pH:8 Temperature: 20 (Room Temperature) |
16 | [2] |
2.20E-02 | mM | Human | Expression Vector: Leukocytes
Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:2°C |
256 | [3] |
2.30E-03 | Expression Vector: Leukocytes
Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:37°C |
1024 |
Mode (mM) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|
2.40E-03 | 5.77E+00 | -5.51E+00 | 7.20E-01 |
Kmp
Mode (mM) | Location parameter (µ) | Scale parameter (σ) |
---|---|---|
2.40E-03 | -5.51E+00 | 7.17E-01 |
kcat
Value | Units | Species | Notes | Weight | Reference |
---|---|---|---|---|---|
12.6 - 28.2 | per minute | Human | Expression Vector: E. Coli.
Enzyme: Leukotriene A4 Hydrolase pH: 7.4 Temperature: 37 °C |
1024 | [4] |
51 | per minute | Human | Expression Vector: E. Coli
Enzyme: Wild Type Leukotriene A4 hydrolase: pH:8 Temperature: 20 (Room Temperature) |
128 | [1] |
90 | per minute | Frog | Expression Vector: oocytes
Enzyme: leukotriene A4 hydrolase pH:8 Temperature: 20 (Room Temperature) |
16 | [2] |
125 | per minute | Human | Expression Vector: Leukocytes
Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:37°C |
1024 | [3] |
Mode (min-1) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|
2.85E+01 | 6.56E+00 | 3.94E+00 | 7.60E-01 |
Enzyme concentration
To convert the enzyme concentration from ppm to mM, the following equation was used.
Value | Units | Species | Notes | Weight | Reference |
---|---|---|---|---|---|
714 | Human | Expression Vector: Lung
Enzyme: LTA4H pH: 7.5 Temperature: 37 °C |
1024 | [5] | |
489 | Human | Expression Vector: Skin
Enzyme: LTA4H pH: 7.5 Temperature: 37 °C |
2048 | [6] | |
410 | Human | Expression Vector: Oral Cavity
Enzyme: LTA4H pH: 7.5 Temperature: 37 °C |
1024 | [6] |
Mode (ppm) | Mode (mM) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|---|
4.88E+02 | 2.70E-03 | 1.25E+00 | 6.24E+00 | 2.19E-01 |
Keq
Gibbs Free Energy Change | Units | Species | Notes | Weight | Reference |
---|---|---|---|---|---|
(-3.6329994) | kcal/mol | Not stated | Estimated
Enzyme: LTA4H Substrate: LTA4 Product: LTB4 pH: 7.3 ionic strength: 0.25 |
64 | [7] |
Mode | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|
4.64E+02 | 1.00E+01 | 6.93E+00 | 8.90E-01 |
References
- ↑ 1.0 1.1 [www.ncbi.nlm.nih.gov/pubmed/8650196 Mueller M. , "Leukotriene A4 hydrolase: protection from mechanism-based inactivation by mutation of tyrosine-378. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5931-5.]
- ↑ 2.0 2.1 [www.ncbi.nlm.nih.gov/pubmed/9744798 Stromberg F. , "Purification and characterization of leukotriene A4 hydrolase from Xenopus laevis oocytes. FEBS Lett. 1998 Aug 21;433(3):219-22.]
- ↑ 3.0 3.1 [www.ncbi.nlm.nih.gov/pubmed/6490615 Radmark O. , "Leukotriene A4 hydrolase in human leukocytes. Purification and properties J Biol Chem. 1984 Oct 25;259(20):12339-45.]
- ↑ [www.ncbi.nlm.nih.gov/pubmed/11675384 Rudberg P. , "Leukotriene A4 Hydrolase/Aminopeptidase The Journal of Biological Chemistry, 277, 1398-1404.]
- ↑ M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
- ↑ 6.0 6.1 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
- ↑ Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471