Transformation of LTA4 to LTB4

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Reaction

Chemical equation

$LTA4 \rightleftharpoons LTB4$

Rate equation

Parameters

Michaelis-Menten Constants

Value	Units	Species	Notes	Reference
5.80E-03	mM	Human	Expression Vector: E. Coli Enzyme: Wild Type Leukotriene A4 hydrolase: pH:8 Temperature: 20 (Room Temperature)
2.70E-03	mM	Frog	Expression Vector: oocytes Enzyme: leukotriene A4 hydrolase pH:8 Temperature: 20 (Room Temperature)
2.20E-02	mM	Human	Expression Vector: Leukocytes Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:2°C
2.30E-03			Expression Vector: Leukocytes Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:37°C

Enzyme Turnover Numbers

Value	Units	Species	Notes	Reference
12.6 - 28.2	per minute	Human	Expression Vector: E. Coli. Enzyme: Leukotriene A4 Hydrolase pH: 7.4 Temperature: 37 °C
51	per minute	Human	Expression Vector: E. Coli Enzyme: Wild Type Leukotriene A4 hydrolase: pH:8 Temperature: 20 (Room Temperature)
90	per minute	Frog	Expression Vector: oocytes Enzyme: leukotriene A4 hydrolase pH:8 Temperature: 20 (Room Temperature)
125	per minute	Human	Expression Vector: Leukocytes Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:37°C

LTA4H Abundance

Value	Units	Species	Notes	Reference
714	$ppm$	Human	Expression Vector: Lung Enzyme: LTA4H pH: 7.5 Temperature: 37 °C
489	$ppm$	Human	Expression Vector: Skin Enzyme: LTA4H pH: 7.5 Temperature: 37 °C
410	$ppm$	Human	Expression Vector: Oral Cavity Enzyme: LTA4H pH: 7.5 Temperature: 37 °C

Gibbs Free Energy Change

Value	Units	Species	Notes	Reference
(-3.6329994)	kcal/mol	Not stated	Estimated Enzyme: LTA4H Substrate: LTA4 Product: LTB4 pH: 7.3 ionic strength: 0.25