Difference between revisions of "Transformation of LTA4 to LTB4"

Revision as of 13:31, 19 August 2016

AA \rightleftharpoons PGH2

Michaelis-Menten Constants
Value	Units	Species	Notes	Reference
5.80E-03	mM	Human	Expression Vector: E. Coli Enzyme: Wild Type Leukotriene A4 hydrolase: pH:8 Temperature: 20 (Room Temperature)	^[1]
2.70E-03	mM	Frog	Expression Vector: oocytes Enzyme: leukotriene A4 hydrolase pH:8 Temperature: 20 (Room Temperature)	^[2]
2.20E-02	mM	Human	Expression Vector: Leukocytes Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:2°C	^[3]
2.30E-03	mM	Human	Expression Vector: Leukocytes Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:37°C	^[3]

Enzyme Turnover Numbers
Value	Units	Species	Notes	Reference
12.6 - 28.2	per minute	Human	Expression Vector: E. Coli. Enzyme: Leukotriene A4 Hydrolase pH: 7.4 Temperature: 37 °C	^[4]
51	per minute	Human	Expression Vector: E. Coli Enzyme: Wild Type Leukotriene A4 hydrolase: pH:8 Temperature: 20 (Room Temperature)	^[1]
90	per minute	Frog	Expression Vector: oocytes Enzyme: leukotriene A4 hydrolase pH:8 Temperature: 20 (Room Temperature)	^[2]
125	per minute	Human	Expression Vector: Leukocytes Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:37°C	^[3]

LTA4H Abundance
Value	Units	Species	Notes	Reference
714	$ppm$	Human	Expression Vector: Lung Enzyme: LTA4H pH: 7.5 Temperature: 37 °C	^[5]
489	$ppm$	Human	Expression Vector: Skin Enzyme: LTA4H pH: 7.5 Temperature: 37 °C	^[6]
410	$ppm$	Human	Expression Vector: Oral Cavity Enzyme: LTA4H pH: 7.5 Temperature: 37 °C	^[6]

Gibbs Free Energy Change
Value	Units	Species	Notes	Reference
(-3.6329994)	kcal/mol	Not stated	Estimated Enzyme: LTA4H Substrate: LTA4 Product: LTB4 pH: 7.3 ionic strength: 0.25	^[7]

↑ ^1.0 ^1.1 [www.ncbi.nlm.nih.gov/pubmed/8650196 Mueller M. , "Leukotriene A4 hydrolase: protection from mechanism-based inactivation by mutation of tyrosine-378. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5931-5.]
↑ ^2.0 ^2.1 [www.ncbi.nlm.nih.gov/pubmed/9744798 Stromberg F. , "Purification and characterization of leukotriene A4 hydrolase from Xenopus laevis oocytes. FEBS Lett. 1998 Aug 21;433(3):219-22.]
↑ ^3.0 ^3.1 [www.ncbi.nlm.nih.gov/pubmed/6490615 Radmark O. , "Leukotriene A4 hydrolase in human leukocytes. Purification and properties J Biol Chem. 1984 Oct 25;259(20):12339-45.]
↑ [www.ncbi.nlm.nih.gov/pubmed/11675384 Rudberg P. , "Leukotriene A4 Hydrolase/Aminopeptidase The Journal of Biological Chemistry, 277, 1398-1404.]
↑ M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
↑ ^6.0 ^6.1 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
↑ Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471

@@ Line 139: / Line 139: @@
 |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 |-
+|}
+{|class="wikitable sortable"
+|+  style="text-align: left;" | Gibbs Free Energy Change
+|-
+! Value
+! Units
+! Species
+! Notes
+! Reference
+|-
+|(-3.6329994)
+|kcal/mol
+|Not stated
+|Estimated
+Enzyme: LTA4H
+Substrate: LTA4
+Product: LTB4
+pH: 7.3
+ionic strength: 0.25
+|<ref name="MetaCyc”>[http://metacyc.org/META/NEW-IMAGE?type=REACTION&object=LEUKOTRIENE-A4-HYDROLASE-RXN Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471]</ref>
 |}