Difference between revisions of "Transformation of LTA4 to LTB4"

From ISMOC
Jump to: navigation, search
(→‎Parameters)
(→‎Parameters)
Line 104: Line 104:
  
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
−
|+  style="text-align: left;" | Enzyme Turnover Numbers
+
|+  style="text-align: left;" | LTA4H Abundance
 
|-
 
|-
 
! Value
 
! Value
Line 112: Line 112:
 
! Reference
 
! Reference
 
|-
 
|-
−
|1.43e-04
+
|714
−
|mM
+
|<math> ppm </math>
−
|Human
+
|Human  
−
|Expression Vector: E. Coli
+
|Expression Vector: Lung
−
Enzyme: Wild Type Leukotriene A4 hydrolase:
+
Enzyme: LTA4H
−
pH:8
+
pH: 7.5
−
Temperature: 20 (Room Temperature)
+
Temperature: 37 °C
−
Calculated by 20ug of 70kDa protein in 200ul solution.  
+
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
−
|<ref name="Mueller1996"> [www.ncbi.nlm.nih.gov/pubmed/8650196 Mueller M. , "Leukotriene A4 hydrolase: protection from mechanism-based inactivation by mutation of tyrosine-378.'' Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5931-5.]</ref>
+
|-
 +
|489
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Skin
 +
Enzyme: LTA4H
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 
|-
 
|-
−
|7.14E-05 - 1.43E-03
+
|410
−
|mM
+
|<math> ppm </math>
−
|Human
+
|Human  
−
|Expression Vector: E. Coli.
+
|Expression Vector: Oral Cavity
−
Enzyme: Leukotriene A4 Hydrolase
+
Enzyme: LTA4H
−
pH: 7.4
+
pH: 7.5
 
Temperature: 37 °C
 
Temperature: 37 °C
−
|<ref name="Rudberg2002"> [www.ncbi.nlm.nih.gov/pubmed/11675384 Rudberg P. , "Leukotriene A4 Hydrolase/Aminopeptidase'' The Journal of Biological Chemistry, 277, 1398-1404.]</ref>  
+
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 
|-
 
|-
 
|}
 
|}

Revision as of 12:58, 12 August 2016

Return to overview

Reaction

R15 LTA4 - LTB4.jpg

Chemical equation

AA \rightleftharpoons PGH2

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
5.80E-03 mM Human Expression Vector: E. Coli

Enzyme: Wild Type Leukotriene A4 hydrolase: pH:8 Temperature: 20 (Room Temperature)

[1]
2.70E-03 mM Frog Expression Vector: oocytes

Enzyme: leukotriene A4 hydrolase pH:8 Temperature: 20 (Room Temperature)

[2]
2.20E-02 mM Human Expression Vector: Leukocytes

Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:2°C

[3]
2.30E-03 Expression Vector: Leukocytes

Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:37°C

Enzyme Turnover Numbers
Value Units Species Notes Reference
12.6 - 28.2 per minute Human Expression Vector: E. Coli.

Enzyme: Leukotriene A4 Hydrolase pH: 7.4 Temperature: 37 °C

[4]
51 per minute Human Expression Vector: E. Coli

Enzyme: Wild Type Leukotriene A4 hydrolase: pH:8 Temperature: 20 (Room Temperature)

[1]
90 per minute Frog Expression Vector: oocytes

Enzyme: leukotriene A4 hydrolase pH:8 Temperature: 20 (Room Temperature)

[2]
125 per minute Human Expression Vector: Leukocytes

Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:37°C

[3]
LTA4H Abundance
Value Units Species Notes Reference
714 ppm Human Expression Vector: Lung

Enzyme: LTA4H pH: 7.5 Temperature: 37 °C

[5]
489 ppm Human Expression Vector: Skin

Enzyme: LTA4H pH: 7.5 Temperature: 37 °C

[6]
410 ppm Human Expression Vector: Oral Cavity

Enzyme: LTA4H pH: 7.5 Temperature: 37 °C

[6]

References

  1. ↑ 1.0 1.1 [www.ncbi.nlm.nih.gov/pubmed/8650196 Mueller M. , "Leukotriene A4 hydrolase: protection from mechanism-based inactivation by mutation of tyrosine-378. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5931-5.]
  2. ↑ 2.0 2.1 [www.ncbi.nlm.nih.gov/pubmed/9744798 Stromberg F. , "Purification and characterization of leukotriene A4 hydrolase from Xenopus laevis oocytes. FEBS Lett. 1998 Aug 21;433(3):219-22.]
  3. ↑ 3.0 3.1 [www.ncbi.nlm.nih.gov/pubmed/6490615 Radmark O. , "Leukotriene A4 hydrolase in human leukocytes. Purification and properties J Biol Chem. 1984 Oct 25;259(20):12339-45.]
  4. ↑ [www.ncbi.nlm.nih.gov/pubmed/11675384 Rudberg P. , "Leukotriene A4 Hydrolase/Aminopeptidase The Journal of Biological Chemistry, 277, 1398-1404.]
  5. ↑ M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  6. ↑ 6.0 6.1 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587

Related Reactions

Retrieved from ‘http://www.systemsbiology.ls.manchester.ac.uk/wiki/index.php?title=Transformation_of_LTA4_to_LTB4&oldid=4682’