Difference between revisions of "Transformation of LTA4 to LTB4"

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(→‎Parameters)
(→‎Parameters)
Line 24: Line 24:
 
|mM
 
|mM
 
|Human
 
|Human
−
|Wild Type, pH 8.0, at room temperature.
+
|Expression Vector: E. Coli
 +
Enzyme: Wild Type Leukotriene A4 hydrolase:
 +
pH:8
 +
Temperature: 20 (Room Temperature)
 
|<ref name="Mueller1996"> [www.ncbi.nlm.nih.gov/pubmed/8650196 Mueller M. , "Leukotriene A4 hydrolase: protection from mechanism-based inactivation by mutation of tyrosine-378.'' Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5931-5.]</ref>
 
|<ref name="Mueller1996"> [www.ncbi.nlm.nih.gov/pubmed/8650196 Mueller M. , "Leukotriene A4 hydrolase: protection from mechanism-based inactivation by mutation of tyrosine-378.'' Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5931-5.]</ref>
 
|-
 
|-
 
|2.70E-03
 
|2.70E-03
 
|mM
 
|mM
−
|Human
+
|Frog
−
|Leukocytes, pH 8.0 at room temperature.
+
|Expression Vector: oocytes
 +
Enzyme: leukotriene  A4  hydrolase
 +
pH:8
 +
Temperature: 20 (Room Temperature)
 
|<ref name="Stromberg1998"> [www.ncbi.nlm.nih.gov/pubmed/9744798 Stromberg F. , "Purification and characterization of leukotriene A4 hydrolase from Xenopus laevis oocytes.'' FEBS Lett. 1998 Aug 21;433(3):219-22.]</ref>
 
|<ref name="Stromberg1998"> [www.ncbi.nlm.nih.gov/pubmed/9744798 Stromberg F. , "Purification and characterization of leukotriene A4 hydrolase from Xenopus laevis oocytes.'' FEBS Lett. 1998 Aug 21;433(3):219-22.]</ref>
 
|-
 
|-
Line 36: Line 42:
 
|rowspan=2|mM
 
|rowspan=2|mM
 
|rowspan=2|Human
 
|rowspan=2|Human
−
|Leukocyte, 2°C, pH 8.0
+
|Expression Vector: Leukocytes
 +
Enzyme: Leukotriene A4 Hydrolase
 +
pH: 8
 +
Temperature:2°C
 
|rowspan=2|<ref name="Radmark1984"> [www.ncbi.nlm.nih.gov/pubmed/6490615 Radmark O. , "Leukotriene A4 hydrolase in human leukocytes. Purification and properties'' J Biol Chem. 1984 Oct 25;259(20):12339-45.]</ref>
 
|rowspan=2|<ref name="Radmark1984"> [www.ncbi.nlm.nih.gov/pubmed/6490615 Radmark O. , "Leukotriene A4 hydrolase in human leukocytes. Purification and properties'' J Biol Chem. 1984 Oct 25;259(20):12339-45.]</ref>
 
|-
 
|-
 
|2.30E-03
 
|2.30E-03
−
|Leukocyte, 37°C, pH 8.0
+
|Expression Vector: Leukocytes
 +
Enzyme: Leukotriene A4 Hydrolase
 +
pH: 8
 +
Temperature:37°C
 
|-
 
|-
 
|}
 
|}
Line 56: Line 68:
 
|per minute
 
|per minute
 
|Human
 
|Human
−
|Wild type enzyme, pH 7.4, 37 °C.
+
|Expression Vector: E. Coli.
 +
Enzyme: Leukotriene A4 Hydrolase
 +
pH: 7.4
 +
Temperature: 37 °C
 
|<ref name="Rudberg2002"> [www.ncbi.nlm.nih.gov/pubmed/11675384 Rudberg P. , "Leukotriene A4 Hydrolase/Aminopeptidase'' The Journal of Biological Chemistry, 277, 1398-1404.]</ref>  
 
|<ref name="Rudberg2002"> [www.ncbi.nlm.nih.gov/pubmed/11675384 Rudberg P. , "Leukotriene A4 Hydrolase/Aminopeptidase'' The Journal of Biological Chemistry, 277, 1398-1404.]</ref>  
 
|-
 
|-
Line 62: Line 77:
 
|per minute
 
|per minute
 
|Human
 
|Human
−
|Wild Type, pH 8.0, at room temperature.
+
|Expression Vector: E. Coli
 +
Enzyme: Wild Type Leukotriene A4 hydrolase:
 +
pH:8
 +
Temperature: 20 (Room Temperature)
 
|<ref name="Mueller1996"> [www.ncbi.nlm.nih.gov/pubmed/8650196 Mueller M. , "Leukotriene A4 hydrolase: protection from mechanism-based inactivation by mutation of tyrosine-378.'' Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5931-5.]</ref>
 
|<ref name="Mueller1996"> [www.ncbi.nlm.nih.gov/pubmed/8650196 Mueller M. , "Leukotriene A4 hydrolase: protection from mechanism-based inactivation by mutation of tyrosine-378.'' Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5931-5.]</ref>
 
|-
 
|-
 
|90
 
|90
 
|per minute
 
|per minute
−
|Human
+
|Frog
−
|Leukocytes, pH 8.0 at room temperature.
+
|Expression Vector: oocytes
 +
Enzyme: leukotriene  A4  hydrolase
 +
pH:8
 +
Temperature: 20 (Room Temperature)
 
|<ref name="Stromberg1998"> [www.ncbi.nlm.nih.gov/pubmed/9744798 Stromberg F. , "Purification and characterization of leukotriene A4 hydrolase from Xenopus laevis oocytes.'' FEBS Lett. 1998 Aug 21;433(3):219-22.]</ref>
 
|<ref name="Stromberg1998"> [www.ncbi.nlm.nih.gov/pubmed/9744798 Stromberg F. , "Purification and characterization of leukotriene A4 hydrolase from Xenopus laevis oocytes.'' FEBS Lett. 1998 Aug 21;433(3):219-22.]</ref>
 
|-
 
|-
Line 74: Line 95:
 
|per minute
 
|per minute
 
|Human
 
|Human
−
|Leukcyte pH 8.0, 37 °C
+
|Expression Vector: Leukocytes
 +
Enzyme: Leukotriene A4 Hydrolase
 +
pH: 8
 +
Temperature:37°C
 
|<ref name="Radmark1984"> [www.ncbi.nlm.nih.gov/pubmed/6490615 Radmark O. , "Leukotriene A4 hydrolase in human leukocytes. Purification and properties'' J Biol Chem. 1984 Oct 25;259(20):12339-45.]</ref>
 
|<ref name="Radmark1984"> [www.ncbi.nlm.nih.gov/pubmed/6490615 Radmark O. , "Leukotriene A4 hydrolase in human leukocytes. Purification and properties'' J Biol Chem. 1984 Oct 25;259(20):12339-45.]</ref>
 
|-
 
|-
Line 91: Line 115:
 
|mM
 
|mM
 
|Human
 
|Human
−
|Calculated by 20ug of 70kDa protein in 200ul solution. Wild Type, pH 8.0, at room temperature.
+
|Expression Vector: E. Coli
 +
Enzyme: Wild Type Leukotriene A4 hydrolase:
 +
pH:8
 +
Temperature: 20 (Room Temperature)
 +
Calculated by 20ug of 70kDa protein in 200ul solution.  
 
|<ref name="Mueller1996"> [www.ncbi.nlm.nih.gov/pubmed/8650196 Mueller M. , "Leukotriene A4 hydrolase: protection from mechanism-based inactivation by mutation of tyrosine-378.'' Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5931-5.]</ref>
 
|<ref name="Mueller1996"> [www.ncbi.nlm.nih.gov/pubmed/8650196 Mueller M. , "Leukotriene A4 hydrolase: protection from mechanism-based inactivation by mutation of tyrosine-378.'' Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5931-5.]</ref>
 
|-
 
|-
Line 97: Line 125:
 
|mM
 
|mM
 
|Human
 
|Human
−
|Wild type enzyme, pH 7.4, 37 °C.
+
|Expression Vector: E. Coli.
 +
Enzyme: Leukotriene A4 Hydrolase
 +
pH: 7.4
 +
Temperature: 37 °C
 
|<ref name="Rudberg2002"> [www.ncbi.nlm.nih.gov/pubmed/11675384 Rudberg P. , "Leukotriene A4 Hydrolase/Aminopeptidase'' The Journal of Biological Chemistry, 277, 1398-1404.]</ref>  
 
|<ref name="Rudberg2002"> [www.ncbi.nlm.nih.gov/pubmed/11675384 Rudberg P. , "Leukotriene A4 Hydrolase/Aminopeptidase'' The Journal of Biological Chemistry, 277, 1398-1404.]</ref>  
 
|-
 
|-

Revision as of 14:15, 9 June 2016

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Reaction

R15 LTA4 - LTB4.jpg

Chemical equation

AA \rightleftharpoons PGH2

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
5.80E-03 mM Human Expression Vector: E. Coli

Enzyme: Wild Type Leukotriene A4 hydrolase: pH:8 Temperature: 20 (Room Temperature)

[1]
2.70E-03 mM Frog Expression Vector: oocytes

Enzyme: leukotriene A4 hydrolase pH:8 Temperature: 20 (Room Temperature)

[2]
2.20E-02 mM Human Expression Vector: Leukocytes

Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:2°C

[3]
2.30E-03 Expression Vector: Leukocytes

Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:37°C

Enzyme Turnover Numbers
Value Units Species Notes Reference
12.6 - 28.2 per minute Human Expression Vector: E. Coli.

Enzyme: Leukotriene A4 Hydrolase pH: 7.4 Temperature: 37 °C

[4]
51 per minute Human Expression Vector: E. Coli

Enzyme: Wild Type Leukotriene A4 hydrolase: pH:8 Temperature: 20 (Room Temperature)

[1]
90 per minute Frog Expression Vector: oocytes

Enzyme: leukotriene A4 hydrolase pH:8 Temperature: 20 (Room Temperature)

[2]
125 per minute Human Expression Vector: Leukocytes

Enzyme: Leukotriene A4 Hydrolase pH: 8 Temperature:37°C

[3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1.43e-04 mM Human Expression Vector: E. Coli

Enzyme: Wild Type Leukotriene A4 hydrolase: pH:8 Temperature: 20 (Room Temperature) Calculated by 20ug of 70kDa protein in 200ul solution.

[1]
7.14E-05 - 1.43E-03 mM Human Expression Vector: E. Coli.

Enzyme: Leukotriene A4 Hydrolase pH: 7.4 Temperature: 37 °C

[4]

References

  1. ↑ 1.0 1.1 1.2 [www.ncbi.nlm.nih.gov/pubmed/8650196 Mueller M. , "Leukotriene A4 hydrolase: protection from mechanism-based inactivation by mutation of tyrosine-378. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5931-5.]
  2. ↑ 2.0 2.1 [www.ncbi.nlm.nih.gov/pubmed/9744798 Stromberg F. , "Purification and characterization of leukotriene A4 hydrolase from Xenopus laevis oocytes. FEBS Lett. 1998 Aug 21;433(3):219-22.]
  3. ↑ 3.0 3.1 [www.ncbi.nlm.nih.gov/pubmed/6490615 Radmark O. , "Leukotriene A4 hydrolase in human leukocytes. Purification and properties J Biol Chem. 1984 Oct 25;259(20):12339-45.]
  4. ↑ 4.0 4.1 [www.ncbi.nlm.nih.gov/pubmed/11675384 Rudberg P. , "Leukotriene A4 Hydrolase/Aminopeptidase The Journal of Biological Chemistry, 277, 1398-1404.]

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