Transformation of AA to 5-HPETE

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5-LOX activity in the skin remains a mystery. The enzyme has a low levels of activity in cutaneous cells, but products of the pathway such as leukotrienes and 5-HETE are always detected in human skin tissue (Breton, Woolf et al. 1996). As with various prostaglandins found in the compartment, it has been suggested that the detected 5-LOX metabolites are as a result of infiltrating cells from the vascular compartment (Kowal-Bielecka, Distler et al. 2001, Kanaoka and Boyce 2004).

Reaction

Chemical equation

$PGH2 \rightleftharpoons 5-HPETE$

Rate equation

5-LOX Parameters

Michaelis-Menten Constants

Value	Units	Species	Notes	Reference
5.10E-03	$mM$	Human	Expression Vector: Baculovirus, Sf9 insect cells Enzyme: Recombinant 5-Lipoxygenase pH: 5.6 Temperature:37
1.20E-02	$mM$	Human	Expression Vector: Polymorphonuclear Leukocytes Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22
6.31E-02	$mM$	Human	Expression Vector: Polymorphonuclear Leukocytes Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22

Enzyme Turnover Numbers

Value	Units	Species	Notes	Reference
1500 + 75	per minute	Potato	Expression Vector:Potato Tubers Enzyme: 5-Lipoxygenase pH:5.5 Temperature: 23

5-LOX Abundance

Value	Units	Species	Notes	Reference
97.3	$ppm$	Human	Expression Vector: Lung Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C
49.8	$ppm$	Human	Expression Vector: Esophagus Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C
31.9	$ppm$	Human	Expression Vector: Oral Cavity Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C

Gibbs Free Energy Change

Value	Units	Species	Notes	Reference
(-15.9) - 18.01	kcal/mol	Soybean	Expression Vector: Soybean Enzyme: Lipoxygenase-1 pH:Not stated Temperature: Not stated
(-69.979996)	kcal/mol	Not stated	Estimated Enzyme: 5-Lipoxygenase Substrate: Arachidonate Product: 5-HPETE pH: 7.3 ionic strength: 0.25