Transformation of AA to 5-HPETE
Contents
Reaction
Chemical equation
Rate equation
5-LOX Parameters
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 5.10E-03 | 
|
Human | Expression Vector: Baculovirus, Sf9 insect cells
Enzyme: Recombinant 5-Lipoxygenase pH: 5.6 Temperature:37 |
[1] |
| 1.20E-02 |
|
Human | Expression Vector: Polymorphonuclear Leukocytes
Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22 |
[2] |
| 6.31E-02 |
|
Human | Expression Vector: Polymorphonuclear Leukocytes
Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22 |
[3] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 1500 + 75 | per minute | Potato | Expression Vector:Potato Tubers
Enzyme: 5-Lipoxygenase pH:5.5 Temperature: 23 |
[4] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 5.19e-03 | mM | Human | Calculated by 0.4mg of 77kDa of protein in 1 mL.
Leukocytes |
[5] |
References
- ↑ Shirumalla R. K. “RBx 7,796: A novel inhibitor of 5-lipoxygenase.” Inflamm Res. 2006 Dec ; 55 (12) : 517-27.
- ↑ Soberman R. J. "5- and 15(omega-6)-lipoxygenases from human polymorphonuclear leukocytes. Methods Enzymol. 1988; 163:344-9.
- ↑ Soberman R. J. “Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid 15-lipoxygenase) of human polymorphonuclear leukocytes.” J Biol Chem. 1985 Apr 10;260(7):4508-15.
- ↑ Mulliez E., “5-Lipoxygenase from potato tubers. Improved purification and physicochemical characteristics” Biochimica et Biophysica Acta, 1987;916(1):13-23.
- ↑ 00000aab0f26&acdnat=1461759858_b7fd721a8b568158e0c0a4843425c1e4 Rouzer C., “Inhibition of human leukocyte 5-lipoxygenase by a 4-hydroxybenzofuran, L-656,224: Evidence for enzyme reduction and inhibitor degradation” Biochemical Pharmacology, Volume 41, Issue 9, 1 May 1991, Pages 1365-1373
