Transformation of AA to 5-HPETE

From ISMOC
Revision as of 13:32, 27 April 2016 by Muttley93 (talk | contribs) (→‎5-LOX Parameters)
Jump to: navigation, search

Return to overview

Reaction

R11 AA - HPETE5.jpg

Chemical equation

AA \rightleftharpoons PGH2

Rate equation

5-LOX Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
5.10E-03 mM Human Recombinant 5-Lipoxygenase [1]
1.20E-02 mM Human PMN Leukocyte [2]
6.31E-02 mM Human Polymorphonuclear Leukocytes [3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1500 + 75 per minute Potato Potato Tubers, pH 5.5, [4]
Michaelis-Menten Constants
Value Units Species Notes Reference
5.19e-03 mM Human Calculated by 0.4mg of 77kDa of protein in 1 mL.

Leukocytes

[5]

References

  1. ↑ Shirumalla R. K. “RBx 7,796: A novel inhibitor of 5-lipoxygenase.” Inflamm Res. 2006 Dec ; 55 (12) : 517-27.
  2. ↑ Soberman R. J. "5- and 15(omega-6)-lipoxygenases from human polymorphonuclear leukocytes. Methods Enzymol. 1988; 163:344-9.
  3. ↑ Soberman R. J. “Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid 15-lipoxygenase) of human polymorphonuclear leukocytes.” J Biol Chem. 1985 Apr 10;260(7):4508-15.
  4. ↑ Mulliez E., “5-Lipoxygenase from potato tubers. Improved purification and physicochemical characteristics” Biochimica et Biophysica Acta, 1987;916(1):13-23.
  5. ↑ 00000aab0f26&acdnat=1461759858_b7fd721a8b568158e0c0a4843425c1e4 Rouzer C., “Inhibition of human leukocyte 5-lipoxygenase by a 4-hydroxybenzofuran, L-656,224: Evidence for enzyme reduction and inhibitor degradation” Biochemical Pharmacology, Volume 41, Issue 9, 1 May 1991, Pages 1365-1373


Related Reactions

Retrieved from ‘http://www.systemsbiology.ls.manchester.ac.uk/wiki/index.php?title=Transformation_of_AA_to_5-HPETE&oldid=4461’