Difference between revisions of "Transformation of AA to 5-HPETE"

Revision as of 12:03, 9 August 2016

PGH2 \rightleftharpoons 5-HPETE

Michaelis-Menten Constants
Value	Units	Species	Notes	Reference
5.10E-03	$mM$	Human	Expression Vector: Baculovirus, Sf9 insect cells Enzyme: Recombinant 5-Lipoxygenase pH: 5.6 Temperature:37	^[1]
1.20E-02	$mM$	Human	Expression Vector: Polymorphonuclear Leukocytes Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22	^[2]
6.31E-02	$mM$	Human	Expression Vector: Polymorphonuclear Leukocytes Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22	^[3]

Enzyme Turnover Numbers
Value	Units	Species	Notes	Reference
1500 + 75	per minute	Potato	Expression Vector:Potato Tubers Enzyme: 5-Lipoxygenase pH:5.5 Temperature: 23	^[4]

5-LOX Abundance
Value	Units	Species	Notes	Reference
97.3	$ppm$	Human	Expression Vector: Lung Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C	^[5]
49.8	$ppm$	Human	Expression Vector: Esophagus Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C	^[6]
31.9	$ppm$	Human	Expression Vector: Oral Cavity Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C	^[6]

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-{|class="wikitable sortable"
-|+  style="text-align: left;" | Michaelis-Menten Constants
-|-
-! Value
-! Units
-! Species
-! Notes
-! Reference
-|-
-|5.19e-03
-|mM
-|Human
-|Calculated by 0.4mg of 77kDa of protein in 1 mL.
-Leukocytes
-|<ref name="Rouzer1990”>[http://ac.els-cdn.com/000629529190110Q/1-s2.0-000629529190110Q-main.pdf?_tid=8b9511da-0c72-11e6-b92e 00000aab0f26&acdnat=1461759858_b7fd721a8b568158e0c0a4843425c1e4 Rouzer C., “Inhibition of human leukocyte 5-lipoxygenase by a 4-hydroxybenzofuran, L-656,224: Evidence for enzyme reduction and inhibitor degradation” Biochemical Pharmacology, Volume 41, Issue 9, 1 May 1991, Pages 1365-1373]</ref>
-|}
 {|class="wikitable sortable"