Difference between revisions of "Transformation of AA to 5-HPETE"

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(→‎5-LOX Parameters)
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Leukocytes
 
Leukocytes
 
|<ref name="Rouzer1990”>[http://ac.els-cdn.com/000629529190110Q/1-s2.0-000629529190110Q-main.pdf?_tid=8b9511da-0c72-11e6-b92e 00000aab0f26&acdnat=1461759858_b7fd721a8b568158e0c0a4843425c1e4 Rouzer C., “Inhibition of human leukocyte 5-lipoxygenase by a 4-hydroxybenzofuran, L-656,224: Evidence for enzyme reduction and inhibitor degradation” Biochemical Pharmacology, Volume 41, Issue 9, 1 May 1991, Pages 1365-1373]</ref>  
 
|<ref name="Rouzer1990”>[http://ac.els-cdn.com/000629529190110Q/1-s2.0-000629529190110Q-main.pdf?_tid=8b9511da-0c72-11e6-b92e 00000aab0f26&acdnat=1461759858_b7fd721a8b568158e0c0a4843425c1e4 Rouzer C., “Inhibition of human leukocyte 5-lipoxygenase by a 4-hydroxybenzofuran, L-656,224: Evidence for enzyme reduction and inhibitor degradation” Biochemical Pharmacology, Volume 41, Issue 9, 1 May 1991, Pages 1365-1373]</ref>  
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{|class="wikitable sortable"
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|+  style="text-align: left;" | 5-LOX Abundance
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|-
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! Value
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! Units
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! Species
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! Notes
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! Reference
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|-
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|97.3
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|<math> ppm </math>
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|Human
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|Expression Vector: Lung
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Enzyme: 5-LOX
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pH: 7.5
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Temperature: 37 °C
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|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
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|-
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|49.8
 +
|<math> ppm </math>
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|Human
 +
|Expression Vector: Esophagus
 +
Enzyme: 5-LOX
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
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|-
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|31.9
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Oral Cavity
 +
Enzyme: 5-LOX
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
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|-
 
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Revision as of 12:03, 9 August 2016

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Reaction

R11 AA - HPETE5.jpg

Chemical equation

PGH2 \rightleftharpoons 5-HPETE

Rate equation

5-LOX Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
5.10E-03 mM Human Expression Vector: Baculovirus, Sf9 insect cells

Enzyme: Recombinant 5-Lipoxygenase pH: 5.6 Temperature:37

[1]
1.20E-02 mM Human Expression Vector: Polymorphonuclear Leukocytes

Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22

[2]
6.31E-02 mM Human Expression Vector: Polymorphonuclear Leukocytes

Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22

[3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1500 + 75 per minute Potato Expression Vector:Potato Tubers

Enzyme: 5-Lipoxygenase pH:5.5 Temperature: 23

[4]
Michaelis-Menten Constants
Value Units Species Notes Reference
5.19e-03 mM Human Calculated by 0.4mg of 77kDa of protein in 1 mL.

Leukocytes

[5]
5-LOX Abundance
Value Units Species Notes Reference
97.3 ppm Human Expression Vector: Lung

Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C

[6]
49.8 ppm Human Expression Vector: Esophagus

Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C

[7]
31.9 ppm Human Expression Vector: Oral Cavity

Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C

[7]

References

  1. ↑ Shirumalla R. K. “RBx 7,796: A novel inhibitor of 5-lipoxygenase.” Inflamm Res. 2006 Dec ; 55 (12) : 517-27.
  2. ↑ Soberman R. J. "5- and 15(omega-6)-lipoxygenases from human polymorphonuclear leukocytes. Methods Enzymol. 1988; 163:344-9.
  3. ↑ Soberman R. J. “Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid 15-lipoxygenase) of human polymorphonuclear leukocytes.” J Biol Chem. 1985 Apr 10;260(7):4508-15.
  4. ↑ Mulliez E., “5-Lipoxygenase from potato tubers. Improved purification and physicochemical characteristics” Biochimica et Biophysica Acta, 1987;916(1):13-23.
  5. ↑ 00000aab0f26&acdnat=1461759858_b7fd721a8b568158e0c0a4843425c1e4 Rouzer C., “Inhibition of human leukocyte 5-lipoxygenase by a 4-hydroxybenzofuran, L-656,224: Evidence for enzyme reduction and inhibitor degradation” Biochemical Pharmacology, Volume 41, Issue 9, 1 May 1991, Pages 1365-1373
  6. ↑ M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  7. ↑ 7.0 7.1 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587


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