Difference between revisions of "Transformation of AA to 5-HPETE"
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| Line 26: | Line 26: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | |Recombinant 5-Lipoxygenase | + | |Expression Vector: Baculovirus, Sf9 insect cells |
| + | Enzyme: Recombinant 5-Lipoxygenase | ||
| + | pH: 5.6 | ||
| + | Temperature:37 | ||
|<ref name="Shirumalla2006”>[http://www.ncbi.nlm.nih.gov/pubmed/17039282 Shirumalla R. K. “RBx 7,796: A novel inhibitor of 5-lipoxygenase.” Inflamm Res. 2006 Dec ; 55 (12) : 517-27.]</ref> | |<ref name="Shirumalla2006”>[http://www.ncbi.nlm.nih.gov/pubmed/17039282 Shirumalla R. K. “RBx 7,796: A novel inhibitor of 5-lipoxygenase.” Inflamm Res. 2006 Dec ; 55 (12) : 517-27.]</ref> | ||
|- | |- | ||
| Line 32: | Line 35: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | | | + | |Expression Vector: Polymorphonuclear Leukocytes |
| + | Enzyme: 5-Lipoxygenase | ||
| + | pH:7.5 | ||
| + | Temperature: 22 | ||
|<ref name="Soberman1988"> [http://www.ncbi.nlm.nih.gov/pubmed/3070300 Soberman R. J. "5- and 15(omega-6)-lipoxygenases from human polymorphonuclear leukocytes.'' Methods Enzymol. 1988; 163:344-9.]</ref> | |<ref name="Soberman1988"> [http://www.ncbi.nlm.nih.gov/pubmed/3070300 Soberman R. J. "5- and 15(omega-6)-lipoxygenases from human polymorphonuclear leukocytes.'' Methods Enzymol. 1988; 163:344-9.]</ref> | ||
|- | |- | ||
| Line 38: | Line 44: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | |Polymorphonuclear Leukocytes | + | |Expression Vector: Polymorphonuclear Leukocytes |
| + | Enzyme: 5-Lipoxygenase | ||
| + | pH:7.5 | ||
| + | Temperature: 22 | ||
|<ref name="Soberman1985”>[http://www.ncbi.nlm.nih.gov/pubmed/3920219 Soberman R. J. “Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid 15-lipoxygenase) of human polymorphonuclear leukocytes.” J Biol Chem. 1985 Apr 10;260(7):4508-15.]</ref> | |<ref name="Soberman1985”>[http://www.ncbi.nlm.nih.gov/pubmed/3920219 Soberman R. J. “Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid 15-lipoxygenase) of human polymorphonuclear leukocytes.” J Biol Chem. 1985 Apr 10;260(7):4508-15.]</ref> | ||
|- | |- | ||
| Line 55: | Line 64: | ||
|per minute | |per minute | ||
|Potato | |Potato | ||
| − | |Potato Tubers | + | |Expression Vector:Potato Tubers |
| + | Enzyme: 5-Lipoxygenase | ||
| + | pH:5.5 | ||
| + | Temperature: 23 | ||
|<ref name="Mulliez1987”>[http://www.sciencedirect.com/science/article/pii/0167483887902056 Mulliez E., “5-Lipoxygenase from potato tubers. Improved purification and physicochemical characteristics” Biochimica et Biophysica Acta, 1987;916(1):13-23.]</ref> | |<ref name="Mulliez1987”>[http://www.sciencedirect.com/science/article/pii/0167483887902056 Mulliez E., “5-Lipoxygenase from potato tubers. Improved purification and physicochemical characteristics” Biochimica et Biophysica Acta, 1987;916(1):13-23.]</ref> | ||
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Revision as of 11:04, 2 June 2016
Contents
Reaction
Chemical equation
Rate equation
5-LOX Parameters
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 5.10E-03 | 
|
Human | Expression Vector: Baculovirus, Sf9 insect cells
Enzyme: Recombinant 5-Lipoxygenase pH: 5.6 Temperature:37 |
[1] |
| 1.20E-02 |
|
Human | Expression Vector: Polymorphonuclear Leukocytes
Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22 |
[2] |
| 6.31E-02 |
|
Human | Expression Vector: Polymorphonuclear Leukocytes
Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22 |
[3] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 1500 + 75 | per minute | Potato | Expression Vector:Potato Tubers
Enzyme: 5-Lipoxygenase pH:5.5 Temperature: 23 |
[4] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 5.19e-03 | mM | Human | Calculated by 0.4mg of 77kDa of protein in 1 mL.
Leukocytes |
[5] |
References
- ↑ Shirumalla R. K. “RBx 7,796: A novel inhibitor of 5-lipoxygenase.” Inflamm Res. 2006 Dec ; 55 (12) : 517-27.
- ↑ Soberman R. J. "5- and 15(omega-6)-lipoxygenases from human polymorphonuclear leukocytes. Methods Enzymol. 1988; 163:344-9.
- ↑ Soberman R. J. “Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid 15-lipoxygenase) of human polymorphonuclear leukocytes.” J Biol Chem. 1985 Apr 10;260(7):4508-15.
- ↑ Mulliez E., “5-Lipoxygenase from potato tubers. Improved purification and physicochemical characteristics” Biochimica et Biophysica Acta, 1987;916(1):13-23.
- ↑ 00000aab0f26&acdnat=1461759858_b7fd721a8b568158e0c0a4843425c1e4 Rouzer C., “Inhibition of human leukocyte 5-lipoxygenase by a 4-hydroxybenzofuran, L-656,224: Evidence for enzyme reduction and inhibitor degradation” Biochemical Pharmacology, Volume 41, Issue 9, 1 May 1991, Pages 1365-1373
