Transformation of AA to 15-HPETE

Reaction

AA \rightleftharpoons PGH2

Michaelis-Menten Constants
Value	Units	Species	Notes	Reference
3.70E-03 ± 3.00E-04	$mM$	Human	Expression Vector: Epithelium Enzyme: 15-Lipoxygenase pH: 7.5 Temperature: 25	^[1]
5.00E-03	$mM$	Human	Expression Vector: Reticulocyte Enzyme: 15-Lipoxygenase pH:7.5 Temperature: Unspecified	^[2]
1.06E-02	$mM$	Human	Expression Vector: Keratinocyte Enzyme: 15-Lipoxygenase pH: 6.7 - 7.3 Temperature: 37	^[3]
1.17E-02 ± 9.00E-04	$mM$	Human	Expression Vector: Baculovirus Insect Cell Enzyme: Wildtype 15-Lipoxygenase pH: 6.8 Temperature: 37	^[4]

Enzyme Turnover Numbers
Value	Units	Species	Notes	Reference
595.8 ± 16.8	$minute^{-1}$	Human	Expression Vector: Epithelium Enzyme: 15-Lipoxygenase pH: 7.5 Temperature: 25	^[1]
37.2 ± 1.8	per minute	Expression Vector:Human prostate epithelial Enzyme:15-lipoxygenase-2	pH 7, Temperature: 22	^[5]
45 ± 1.2			pH 7.5, Temperature: 22
44.4 ± 2.4			pH 8, Temperature: 22
34.2 ± 0.6			15°C, pH = 7.5
45 ± 1.2			22°C, pH = 7.5
62.4 ± 4.2			30°C, pH = 7.5
82.8 ± 4.8			37°C, pH = 7.5

15-LOX Abundance
Value	Units	Species	Notes	Reference
4.09	$ppm$	Human	Expression Vector: Lung Enzyme: 15-LOX pH: 7.5 Temperature: 37 °C	^[6]
37.4	$ppm$	Human	Expression Vector: Spleen Enzyme: 15-LOX pH: 7.5 Temperature: 37 °C	^[7]
1.40	$ppm$	Human	Expression Vector: Gut Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C	^[6]

Gibbs Free Energy Change
Value	Units	Species	Notes	Reference
(-69.979996)	kcal/mol	Not stated	Estimated Enzyme: 15-LOX Substrate: Arachidonate Product: 15-HPETE pH: 7.3 ionic strength: 0.25	^[8]