Transformation of AA to 15-HPETE

Reaction

AA \rightleftharpoons PGH2

Michaelis-Menten Constants
Value	Units	Species	Notes	Reference
3.70E-03 ± 3.00E-04	$mM$	Human	Expression Vector: Epithelium Enzyme: 15-Lipoxygenase pH: 7.5 Temperature: 25	^[1]
5.00E-03	$mM$	Human	Expression Vector: Reticulocyte Enzyme: 15-Lipoxygenase pH:7.5 Temperature: Unspecified	^[2]
1.06E-02	$mM$	Human	Expression Vector: Keratinocyte Enzyme: 15-Lipoxygenase pH: 6.7 - 7.3 Temperature: 37	^[3]
1.17E-02 ± 9.00E-04	$mM$	Human	Expression Vector: Baculovirus Insect Cell Enzyme: Wildtype 15-Lipoxygenase pH: 6.8 Temperature: 37	^[4]

Enzyme Turnover Numbers
Value	Units	Species	Notes	Reference
595.8 ± 16.8	$minute^{-1}$	Human	Expression Vector: Epithelium Enzyme: 15-Lipoxygenase pH: 7.5 Temperature: 25	^[1]
37.2 ± 1.8	per minute	Expression Vector:Human prostate epithelial Enzyme:15-lipoxygenase-2	pH 7, Temperature: 22	^[5]
45 ± 1.2			pH 7.5, Temperature: 22
44.4 ± 2.4			pH 8, Temperature: 22
34.2 ± 0.6			15°C, pH = 7.5
45 ± 1.2			22°C, pH = 7.5
62.4 ± 4.2			30°C, pH = 7.5
82.8 ± 4.8			37°C, pH = 7.5

Enzyme Concentrations
Value	Units	Species	Notes	Reference
1.60e-05 - 4.00e-05	mM	Human	Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5, 25C), with and without product (12-HETE) addition, in the presence of 25 μM of substrate.	^[5]
2.00e-05	mM	Human	Epithelium	^[1] _