Difference between revisions of "Transformation of AA to 15-HPETE"

Revision as of 15:36, 18 August 2016

AA \rightleftharpoons PGH2

Michaelis-Menten Constants
Value	Units	Species	Notes	Reference
3.70E-03 ± 3.00E-04	$mM$	Human	Expression Vector: Epithelium Enzyme: 15-Lipoxygenase pH: 7.5 Temperature: 25	^[1]
5.00E-03	$mM$	Human	Expression Vector: Reticulocyte Enzyme: 15-Lipoxygenase pH:7.5 Temperature: Unspecified	^[2]
1.06E-02	$mM$	Human	Expression Vector: Keratinocyte Enzyme: 15-Lipoxygenase pH: 6.7 - 7.3 Temperature: 37	^[3]
1.17E-02 ± 9.00E-04	$mM$	Human	Expression Vector: Baculovirus Insect Cell Enzyme: Wildtype 15-Lipoxygenase pH: 6.8 Temperature: 37	^[4]

Enzyme Turnover Numbers
Value	Units	Species	Notes	Reference
595.8 ± 16.8	$minute^{-1}$	Human	Expression Vector: Epithelium Enzyme: 15-Lipoxygenase pH: 7.5 Temperature: 25	^[1]
37.2 ± 1.8	per minute	Expression Vector:Human prostate epithelial Enzyme:15-lipoxygenase-2	pH 7, Temperature: 22	^[5]
45 ± 1.2			pH 7.5, Temperature: 22
44.4 ± 2.4			pH 8, Temperature: 22
34.2 ± 0.6			15°C, pH = 7.5
45 ± 1.2			22°C, pH = 7.5
62.4 ± 4.2			30°C, pH = 7.5
82.8 ± 4.8			37°C, pH = 7.5

15-LOX Abundance
Value	Units	Species	Notes	Reference
4.09	$ppm$	Human	Expression Vector: Lung Enzyme: 15-LOX pH: 7.5 Temperature: 37 °C	^[6]
37.4	$ppm$	Human	Expression Vector: Spleen Enzyme: 15-LOX pH: 7.5 Temperature: 37 °C	^[7]
1.40	$ppm$	Human	Expression Vector: Gut Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C	^[6]

Gibbs Free Energy Change
Value	Units	Species	Notes	Reference
(-69.979996)	kcal/mol	Not stated	Estimated Enzyme: 15-LOX Substrate: Arachidonate Product: 15-HPETE pH: 7.3 ionic strength: 0.25	^[8]

@@ Line 141: / Line 141: @@
 |<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 |-
+|}
+{|class="wikitable sortable"
+|+  style="text-align: left;" | Gibbs Free Energy Change
+|-
+! Value
+! Units
+! Species
+! Notes
+! Reference
+|-
+|(-69.979996)
+|kcal/mol
+|Not stated
+|Estimated
+Enzyme: 15-LOX
+Substrate: Arachidonate
+Product: 15-HPETE
+pH: 7.3
+ionic strength: 0.25
+|<ref name="MetaCyc”>[http://metacyc.org/META/NEW-IMAGE?type=REACTION&object=RXN66-490 Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471]</ref>
 |}