Difference between revisions of "Transformation of AA to 15-HPETE"

Revision as of 11:03, 27 April 2016

AA \rightleftharpoons PGH2

Michaelis-Menten Constants
Value	Units	Species	Notes	Reference
3.70E-03 ± 3.00E-04	$mM$	Human	Epithelium	^[1]
5.00E-03	$mM$	Human	Reticulocyte	^[2]
1.06E-02	$mM$	Human	Keratinocyte	^[3]
1.17E-02 ± 9.00E-04	$mM$	Human	Wildtype 15-Lipoxygenase	^[4]

Enzyme Turnover Numbers
Value	Units	Species	Notes	Reference
595.8 ± 16.8	$minute^{-1}$	Human	Epithelium	^[1]
37.2 ± 1.8	per minute	Human prostate epithelial 15-lipoxygenase-2	pH 7	^[5]
45 ± 1.2			pH 7.5
44.4 ± 2.4			pH 8
34.2 ± 0.6			15°C
45 ± 1.2			22°C
62.4 ± 4.2			30°C
82.8 ± 4.8			37°C

Enzyme Concentrations
Value	Units	Species	Notes	Reference
1.60e-05 - 4.00e-05	mM	Human	Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5, 25C), with and without product (12-HETE) addition, in the presence of 25 μM of substrate.	^[5]
2.00e-05	mM	Human	Epithelium	^[1] _

@@ Line 105: / Line 105: @@
 |<ref name="Wecksler2009"> [www.ncbi.nlm.nih.gov/pubmed/19469483 Wecksler A. "Mechanistic Investigations of Human Reticulocyte 15- and Platelet 12-Lipoxygenases with Arachidonic Acid'' Biochemistry, 2009, 48 (26), pp 6259–6267]</ref>
 |-
+|2.00e-05
+|mM
+|Human
+|Epithelium
+|<ref name="Jacquot2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18547056 Jacquot C. "Isotope sensitive branching and kinetic isotope effects in the reaction of deuterated arachidonic acids with human 12- and 15-lipoxygenases.'' Biochemistry. 2008 Jul 8;47(27):7295-303. doi: 10.1021/bi800308q. Epub 2008 Jun 12.]</ref> _
 |}