Transformation of AA to 12-HPETE

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Reaction

Chemical equation

$AA \rightleftharpoons PGH2$

12-LOX Parameters

Michaelis-Menten Constants

Value	Units	Species	Notes	Reference
7.20E-03	$mM$	Human	Expression Vector: Platelet Enzyme: 12-Lipoxygenase pH: 7.4 Temperature: 37°C.
8.00E-02	$mM$	Human	Expression Vector: Platelet Enzyme: 12-Lipoxygenase pH: 7 Temperature: 24
1.00E-02	$mM$	Human Platlet	Expression Vector: Baculovirus Enzyme: 12-Lipoxygenase pH: 8 Temperature: 37
7.90E-03 ± 8.00E-04	$mM$	Human	Expression Vector: Platelets Enzyme:12-Lipoxygenase pH: 7.4 Temperature: 37

Enzyme Turnover Numbers

Value	Units	Species	Notes	Reference
336 ± 12	per minute	Human	Expression Vector: Human reticulocyte Enzyme: 15-lipoxygenase-1 pH: 7.5 Temperature: 25
504	per minute	Wild Boar	Expression Vector: E. coli. Enzyme: 12-Lipoxygenase pH: 7.4 Temperature: 37

12-LOX Abundance

Value	Units	Species	Notes	Reference
97.3	$ppm$	Human	Expression Vector: Lung Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C
49.8	$ppm$	Human	Expression Vector: Esophagus Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C
31.9	$ppm$	Human	Expression Vector: Oral Cavity Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C