Transformation of AA to 12-HPETE

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Reaction

R179AA - HPETE12.jpg

Chemical equation

AA \rightleftharpoons PGH2

Rate equation

12-LOX Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
7.20E-03 mM Human Platelet [1]
8.00E-02 mM Human Platelet [2]
1.00E-02 mM Human Recombinant Histidine-Tagged Human Platelet 12-Lipoxygenase [3]
7.90E-03 ± 8.00E-04 mM Human Platelet 12-Lipoxygenase [4]
Enzyme Turnover Numbers
Value Units Species Notes Reference
336 ± 12 per minute Human Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5,

25 �C), with and without product (12-HETE) addition, in the presence of 25 μM of substrate.

[5]
504 per minute Wild Boar Wild Type Leukocyte-Type 12-Lipoxygenase Expressed in E. coli. [6]

References

  1. ↑ Lagarde M. "Subcellular localization and some properties of lipoxygenase activity in human blood platelets. Biochem J. 1984 Sep 1;222(2):495-500.
  2. ↑ Hada T. "Catalytic properties of human platelet 12-lipoxygenase as compared with the enzymes of other origins. Biochim Biophys Acta. 1991 Apr 24;1083(1):89-93.
  3. ↑ Chen X. S. "Purification and characterization of recombinant histidine-tagged human platelet 12-lipoxygenase expressed in a baculovirus/insect cell system. Eur J Biochem. 1993 Jun 15;214(3):845-52.
  4. ↑ Romano M. "Lipoxin synthase activity of human platelet 12-lipoxygenase. Biochem J. 1993 Nov 15;296 ( Pt 1):127-33.
  5. ↑ [www.ncbi.nlm.nih.gov/pubmed/19469483 Wecksler A. "Mechanistic Investigations of Human Reticulocyte 15- and Platelet 12-Lipoxygenases with Arachidonic Acid Biochemistry, 2009, 48 (26), pp 6259–6267]
  6. ↑ Richards K. "Leukocyte 12-Lipoxygenase:  Expression, Purification, and Investigation of the Role of Methionine Residues in Turnover-Dependent Inactivation and 5,8,11,14-Eicosatetraynoic Acid Inhibition Biochemistry, 1997, 36 (22), pp 6692–6699

Related Reactions

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