Difference between revisions of "Transformation of AA to 12-HPETE"

Revision as of 15:33, 18 August 2016

AA \rightleftharpoons PGH2

Michaelis-Menten Constants
Value	Units	Species	Notes	Reference
7.20E-03	$mM$	Human	Expression Vector: Platelet Enzyme: 12-Lipoxygenase pH: 7.4 Temperature: 37°C.	^[1]
8.00E-02	$mM$	Human	Expression Vector: Platelet Enzyme: 12-Lipoxygenase pH: 7 Temperature: 24	^[2]
1.00E-02	$mM$	Human Platlet	Expression Vector: Baculovirus Enzyme: 12-Lipoxygenase pH: 8 Temperature: 37	^[3]
7.90E-03 ± 8.00E-04	$mM$	Human	Expression Vector: Platelets Enzyme:12-Lipoxygenase pH: 7.4 Temperature: 37	^[4]

Enzyme Turnover Numbers
Value	Units	Species	Notes	Reference
336 ± 12	per minute	Human	Expression Vector: Human reticulocyte Enzyme: 15-lipoxygenase-1 pH: 7.5 Temperature: 25	^[5]
504	per minute	Wild Boar	Expression Vector: E. coli. Enzyme: 12-Lipoxygenase pH: 7.4 Temperature: 37	^[6]

12-LOX Abundance
Value	Units	Species	Notes	Reference
19.8	$ppm$	Human	Expression Vector: Spleen Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C	^[7]
1.60	$ppm$	Human	Expression Vector: Liver Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C	^[8]
0.28	$ppm$	Human	Expression Vector: Gut Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C	^[8]
0.11	$ppm$	Human	Expression Vector: Pancreas Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C	^[8]

Gibbs Free Energy Change
Value	Units	Species	Notes	Reference
kcal/mol	Not stated	Estimated Enzyme: 12-LOX Substrate: Arachidonate Product: 12-HPETE pH: 7.3 ionic strength: 0.25	^[9]

@@ Line 135: / Line 135: @@
 |<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 |-
+|}
+{|class="wikitable sortable"
+|+  style="text-align: left;" | Gibbs Free Energy Change
+|-
+! Value
+! Units
+! Species
+! Notes
+! Reference
+|-
+|-69.979996
+|kcal/mol
+|Not stated
+|Estimated
+Enzyme: 12-LOX
+Substrate: Arachidonate
+Product: 12-HPETE
+pH: 7.3
+ionic strength: 0.25
+|<ref name="MetaCyc”>[http://metacyc.org/META/NEW-IMAGE?type=REACTION&object=ARACHIDONATE-12-LIPOXYGENASE-RXN Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471]</ref>
 |}