Difference between revisions of "Transformation of AA to 12-HPETE"
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|Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5, | |Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5, | ||
| − | + | 25C), with and without product (12-HETE) addition, in the presence of 25 μM of substrate. | |
|<ref name="Wecksler2009"> [www.ncbi.nlm.nih.gov/pubmed/19469483 Wecksler A. "Mechanistic Investigations of Human Reticulocyte 15- and Platelet 12-Lipoxygenases with Arachidonic Acid'' Biochemistry, 2009, 48 (26), pp 6259–6267]</ref> | |<ref name="Wecksler2009"> [www.ncbi.nlm.nih.gov/pubmed/19469483 Wecksler A. "Mechanistic Investigations of Human Reticulocyte 15- and Platelet 12-Lipoxygenases with Arachidonic Acid'' Biochemistry, 2009, 48 (26), pp 6259–6267]</ref> | ||
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Revision as of 17:47, 10 April 2016
Contents
Reaction
Chemical equation
Rate equation
12-LOX Parameters
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 7.20E-03 | 
|
Human | Platelet | [1] |
| 8.00E-02 |
|
Human | Platelet | [2] |
| 1.00E-02 |
|
Human | Recombinant Histidine-Tagged Human Platelet 12-Lipoxygenase | [3] |
| 7.90E-03 ± 8.00E-04 |
|
Human | Platelet 12-Lipoxygenase | [4] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 336 ± 12 | per minute | Human | Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5,
25C), with and without product (12-HETE) addition, in the presence of 25 μM of substrate. |
[5] |
| 504 | per minute | Wild Boar | Wild Type Leukocyte-Type 12-Lipoxygenase Expressed in E. coli. | [6] |
References
- ↑ Lagarde M. "Subcellular localization and some properties of lipoxygenase activity in human blood platelets. Biochem J. 1984 Sep 1;222(2):495-500.
- ↑ Hada T. "Catalytic properties of human platelet 12-lipoxygenase as compared with the enzymes of other origins. Biochim Biophys Acta. 1991 Apr 24;1083(1):89-93.
- ↑ Chen X. S. "Purification and characterization of recombinant histidine-tagged human platelet 12-lipoxygenase expressed in a baculovirus/insect cell system. Eur J Biochem. 1993 Jun 15;214(3):845-52.
- ↑ Romano M. "Lipoxin synthase activity of human platelet 12-lipoxygenase. Biochem J. 1993 Nov 15;296 ( Pt 1):127-33.
- ↑ [www.ncbi.nlm.nih.gov/pubmed/19469483 Wecksler A. "Mechanistic Investigations of Human Reticulocyte 15- and Platelet 12-Lipoxygenases with Arachidonic Acid Biochemistry, 2009, 48 (26), pp 6259–6267]
- ↑ Richards K. "Leukocyte 12-Lipoxygenase: Expression, Purification, and Investigation of the Role of Methionine Residues in Turnover-Dependent Inactivation and 5,8,11,14-Eicosatetraynoic Acid Inhibition Biochemistry, 1997, 36 (22), pp 6692–6699
