Transformation of 5-HPETE to 5-HETE

From ISMOC
Revision as of 11:11, 27 April 2016 by Muttley93 (talk | contribs) (→‎PHGPx Parameters)
Jump to: navigation, search

Return to overview

Reaction

R12 HPETE5-HETE5.jpg

Chemical equation

AA \rightleftharpoons PGH2

Rate equation

PHGPx Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
3.00E-01 mM Human Bioimprited Enzyme [1]
11.1 ± 2.90E-01 mM Human Wild Type Enzyme (Se-hGSTZ1-1) [2]
4.00E-03 mM Rat Liver cytosol Se-GSH Px (5-HPETE as the substrate) [3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1170 ± 50 min^{-1} Human Bioimprited Enzyme [1]
24500 ± 150 min^{-1} Human Wild Type Enzyme (Se-hGSTZ1-1) [2]
Enzyme Concentrations
Value Units Species Notes Reference
0.0005-0.005 mM Human Bioimprited Enzyme [1]
0.004 mM Human Wild Type Enzyme (Se-hGSTZ1-1) [2]

References

  1. ↑ 1.0 1.1 1.2 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein. Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.
  2. ↑ 2.0 2.1 2.2 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases. Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.
  3. ↑ Hiratsuka A. "Subunit Ya-specific glutathione peroxidase activity toward cholesterol 7-hydroperoxides of glutathione S-transferases in cytosols from rat liver and skin. J Biol Chem. 1997 Feb 21;272(8):4763-9.

Related Reactions

Retrieved from ‘http://www.systemsbiology.ls.manchester.ac.uk/wiki/index.php?title=Transformation_of_5-HPETE_to_5-HETE&oldid=4453’