Difference between revisions of "Transformation of 5-HPETE to 5-HETE"

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[[File:R12.PNG|center|500px]]
 
[[File:R12.PNG|center|500px]]
  
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== PHGPx Parameters ==
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== Enzyme Parameters ==
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=== K<sub>ms</sub> ===
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
−
|+  style="text-align: left;" | Michaelis-Menten Constants
+
|+  style="text-align: left;" | Literature values
 
|-
 
|-
 
! Value
 
! Value
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|}
 
|}
  
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{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the PHGPx Kms distribution
 +
! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 3.19E-01 || 1.02E+03 || 2.54E+00 || 1.92E+00
 +
|}
 +
 +
 +
 +
=== K<sub>mp</sub> ===
 +
This is a “Dependent parameter”, meaning that the log-normal distribution for this parameter was calculated using multivariate distributions  (this is discussed in detail[[Quantification of parameter uncertainty | here]]). As a result, no confidence interval factor or literature values were cited for this parameter. 
 +
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the PHGPx Kmp distribution
 +
! Mode (mM) !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 3.15E-01 || 2.53E+00 || 1.92E+00
 +
|}
 +
 +
=== k<sub>cat</sub> ===
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
−
|+  style="text-align: left;" | Enzyme Turnover Numbers
+
|+  style="text-align: left;" | Literature values
 
|-
 
|-
 
! Value
 
! Value
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|-
 
|-
 
|}
 
|}
 +
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the PHGPx kcat distribution
 +
! Mode (min-1) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 2.44E+04 || 4.20E+00 || 1.11E+01 || 9.75E-01
 +
|}
 +
 +
 +
 +
=== Enzyme concentration ===
  
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
−
|+  style="text-align: left;" | PHGPx Abundance
+
|+  style="text-align: left;" | Literature values
 
|-
 
|-
 
! Value
 
! Value
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|-
 
|-
 
|}
 
|}
 +
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the PHGPx concentration distribution
 +
! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 3.14E+02 || 1.38E+00 || 5.85E+00 || 3.09E-01
 +
|}
 +
 +
 +
 +
=== K<sub>eq</sub> ===
  
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
−
|+  style="text-align: left;" | Gibbs Free Energy Change
+
|+  style="text-align: left;" | Literature values
 
|-
 
|-
−
! Value
+
! Gibbs Free Energy Change
 
! Units
 
! Units
 
! Species
 
! Species
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|<ref name="MetaCyc”>[http://metacyc.org/META/NEW-IMAGE?type=REACTION&object=1.11.1.12-RXN Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471]</ref>
 
|<ref name="MetaCyc”>[http://metacyc.org/META/NEW-IMAGE?type=REACTION&object=1.11.1.12-RXN Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471]</ref>
 
|}
 
|}
 +
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the PHGPx Keq distribution
 +
! Mode !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 5.90E+19 || 1.00E+01 || 4.63E+01 || 8.90E-01
 +
|}
 +
 +
 +
  
 
== References ==
 
== References ==

Revision as of 10:26, 15 May 2019

Return to overview

5-HPETE is reduced by phospholipid hydroperoxide glutathione peroxidase (PHGPx) to the corresponding alcohol, 5-HETE (Suth01).

Reaction

R12 HPETE5-HETE5.jpg

Chemical equation

5-HPETE \rightleftharpoons 5-HETE

Rate equation

R12.PNG

Enzyme Parameters

Kms

Literature values
Value Units Species Notes Reference
3.00E-01 mM Human Expression Vector: Bioimprited Enzyme - Selenosubtilisin

Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37

[1]
11.1 ± 2.90E-01 mM Human Expression Vector: E Coli

Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37

[2]
4.00E-03 mM Rat Expression Vector: Rat Liver Cells

Enzyme: Glutathione Peroxidase pH: 7.4 Temperature: 37

[3]
Description of the PHGPx Kms distribution
Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
3.19E-01 1.02E+03 2.54E+00 1.92E+00


Kmp

This is a “Dependent parameter”, meaning that the log-normal distribution for this parameter was calculated using multivariate distributions (this is discussed in detail here). As a result, no confidence interval factor or literature values were cited for this parameter.

Description of the PHGPx Kmp distribution
Mode (mM) Location parameter (µ) Scale parameter (σ)
3.15E-01 2.53E+00 1.92E+00

kcat

Literature values
Value Units Species Notes Reference
1170 ± 50 min^{-1} Human Expression Vector: Bioimprited Enzyme - Selenosubtilisin

Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37

[1]
24500 ± 150 min^{-1} Human Expression Vector: E Coli

Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37

[2]
Description of the PHGPx kcat distribution
Mode (min-1) Confidence Interval Location parameter (µ) Scale parameter (σ)
2.44E+04 4.20E+00 1.11E+01 9.75E-01


Enzyme concentration

Literature values
Value Units Species Notes Reference
459 ppm Human Expression Vector: Skin

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

[4]
307 ppm Human Expression Vector: Lung

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

[4]
282 ppm Human Expression Vector: Esophagus

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

[4]
204 ppm Human Expression Vector: Gut

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

[5]
Description of the PHGPx concentration distribution
Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
3.14E+02 1.38E+00 5.85E+00 3.09E-01


Keq

Literature values
Gibbs Free Energy Change Units Species Notes Reference
(-26.941177) kcal/mol Not stated Estimated

Enzyme: PHGPx Substrate:a hydroperoxy-fatty-acyl-[lipid] Product: a hydroxy-fatty-acyl-[lipid] pH: 7.3 ionic strength: 0.25

[6]
Description of the PHGPx Keq distribution
Mode Confidence Interval Location parameter (µ) Scale parameter (σ)
5.90E+19 1.00E+01 4.63E+01 8.90E-01



References

  1. ↑ 1.0 1.1 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein. Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.
  2. ↑ 2.0 2.1 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases. Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.
  3. ↑ Hiratsuka A. "Subunit Ya-specific glutathione peroxidase activity toward cholesterol 7-hydroperoxides of glutathione S-transferases in cytosols from rat liver and skin. J Biol Chem. 1997 Feb 21;272(8):4763-9.
  4. ↑ 4.0 4.1 4.2 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
  5. ↑ M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  6. ↑ Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471

Related Reactions

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