Difference between revisions of "Transformation of 5-HPETE to 5-HETE"

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(→‎PHGPx Parameters)
(→‎PHGPx Parameters)
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{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
−
|+  style="text-align: left;" | Enzyme Concentrations
+
|+  style="text-align: left;" | PHGPx Abundance
 
|-
 
|-
 
! Value
 
! Value
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! Reference
 
! Reference
 
|-
 
|-
−
|0.0005-0.005
+
|459
−
|mM
+
|<math> ppm </math>
 
|Human  
 
|Human  
−
|Expression Vector: Bioimprited Enzyme - Selenosubtilisin
+
|Expression Vector: Skin
−
Enzyme: Glutathione Peroxidase
+
Enzyme: PHGPx
−
pH: 7
+
pH: 7.5
−
Temperature: 37  
+
Temperature: 37 °C
−
|<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref>
+
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]
 
|-
 
|-
−
|0.004
+
|307
−
|mM
+
|<math> ppm </math>
 
|Human  
 
|Human  
−
|Expression Vector: E Coli
+
|Expression Vector: Lung
−
Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1)
+
Enzyme: PHGPx
−
pH: 7
+
pH: 7.5
−
Temperature: 37
+
Temperature: 37 °C
−
|<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref>  
+
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]
 +
 
 +
</ref><ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 +
|-
 +
|282
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Esophagus
 +
Enzyme: PHGPx
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 +
|-
 +
|204
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Gut
 +
Enzyme: PHGPx
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|</ref><ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|}
 
|}
  

Revision as of 11:57, 12 August 2016

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R12 HPETE5-HETE5.jpg

Chemical equation

AA \rightleftharpoons PGH2

Rate equation

PHGPx Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
3.00E-01 mM Human Expression Vector: Bioimprited Enzyme - Selenosubtilisin

Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37

[1]
11.1 ± 2.90E-01 mM Human Expression Vector: E Coli

Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37

[2]
4.00E-03 mM Rat Expression Vector: Rat Liver Cells

Enzyme: Glutathione Peroxidase pH: 7.4 Temperature: 37

[3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1170 ± 50 min^{-1} Human Expression Vector: Bioimprited Enzyme - Selenosubtilisin

Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37

[1]
24500 ± 150 min^{-1} Human Expression Vector: E Coli

Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37

[2]
PHGPx Abundance
Value Units Species Notes Reference
459 ppm Human Expression Vector: Skin

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

Cite error: Closing </ref> missing for <ref> tag[4]
282 ppm Human Expression Vector: Esophagus

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

[5]
204 ppm Human Expression Vector: Gut

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

</ref>[4]

References

  1. ↑ 1.0 1.1 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein. Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.
  2. ↑ 2.0 2.1 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases. Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.
  3. ↑ Hiratsuka A. "Subunit Ya-specific glutathione peroxidase activity toward cholesterol 7-hydroperoxides of glutathione S-transferases in cytosols from rat liver and skin. J Biol Chem. 1997 Feb 21;272(8):4763-9.
  4. ↑ 4.0 4.1 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  5. ↑ M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587

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