Difference between revisions of "Transformation of 5-HPETE to 5-HETE"

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! Reference
 
! Reference
 
|-
 
|-
−
 
+
|3.00E-01
 +
|<math> mM </math>
 +
|Human
 +
|Bioimprited Enzyme
 +
|<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref>
 +
|-
 +
|11.1 ± 2.90E-01
 +
|<math> mM </math>
 +
|Human
 +
|Wild Type Enzyme (Se-hGSTZ1-1)
 +
|<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref>
 +
|-
 +
|4.00E-03
 +
|<math> mM </math>
 +
|Rat
 +
|Liver cytosol Se-GSH Px (5-HPETE as the substrate)
 +
|<ref name="Hirat1997"> [http://www.ncbi.nlm.nih.gov/pubmed/9030530 Hiratsuka A. "Subunit Ya-specific glutathione peroxidase activity toward cholesterol 7-hydroperoxides of glutathione S-transferases in cytosols from rat liver and skin.'' J Biol Chem. 1997 Feb 21;272(8):4763-9.]</ref>
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! Reference
 
! Reference
 
|-
 
|-
−
 
+
|1170 ± 50
−
 
+
|<math> min^{-1} </math>
 +
|Human
 +
|Bioimprited Enzyme
 +
|<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref>
 +
|-
 +
|24500 ± 150
 +
|<math> min^{-1} </math>
 +
|Human
 +
|Wild Type Enzyme (Se-hGSTZ1-1)
 +
|<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref>
 +
|-
 
|}
 
|}
  

Revision as of 13:54, 8 April 2016

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Reaction

R12 HPETE5-HETE5.jpg

Chemical equation

AA \rightleftharpoons PGH2

Rate equation

PHGPx Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
3.00E-01 mM Human Bioimprited Enzyme [1]
11.1 ± 2.90E-01 mM Human Wild Type Enzyme (Se-hGSTZ1-1) [2]
4.00E-03 mM Rat Liver cytosol Se-GSH Px (5-HPETE as the substrate) [3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1170 ± 50 min^{-1} Human Bioimprited Enzyme [1]
24500 ± 150 min^{-1} Human Wild Type Enzyme (Se-hGSTZ1-1) [2]

References

  1. ↑ 1.0 1.1 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein. Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.
  2. ↑ 2.0 2.1 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases. Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.
  3. ↑ Hiratsuka A. "Subunit Ya-specific glutathione peroxidase activity toward cholesterol 7-hydroperoxides of glutathione S-transferases in cytosols from rat liver and skin. J Biol Chem. 1997 Feb 21;272(8):4763-9.

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