Transformation of 15-Keto-PGE2 to 13,14-Dihydro-15-Keto-PGE2

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Reaction

Chemical equation

Failed to parse (Cannot store math image on filesystem.): 15-Keto-PGE2 \rightleftharpoons 13,14-Dihydro-15-Keto-PGE2

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
0.0006 mM Unkown Follows a ping-pong mechanism by binding with NADP+, transforming it to NADPH and releasing it before binding 5S-HETE. [1]
0.00067 mM Human Cell lines Follows a ping-pong mechanism by binding with NADP+ (Km 139 nM), transforming it to NADPH (inhibited by NADPH (Ki 224 nM)) and releasing it before binding 5S-HETE.

Method: In vitro Organism: Human Expression vector: U937 and HL-60 cell lines Enzyme:5-HEDH pH: 7.4 Temperature: 37 ◦C

[2]
0.000516 ± 0.00019 mM Human Cell lines

Method: In vitro Organism: Human Expression vector: U937 Enzyme:5-HEDH pH: 7.4 Temperature: 37 ◦C

[3]
Michaelis-Menten Constants
Value Units Species Notes Reference
X Y Z A B
Enzyme Turnover Number
Value Units Species Notes Reference
X Y Z A B
5-HEDH Abundance
Value Units Species Notes Reference
X Y Z A B
Gibbs Free energy
Value Units Species Notes Reference
X Y Z A B


Half Life
Value Units Species Notes Reference
9.9 +/-0.2 minutes Dog In vivo

Temperature:37 pH:7

[4]
  1. ↑ Lipoxygenases in Inflammation - edited by Dieter Steinhilber
  2. ↑ [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828885/pdf/bj4030157.pdf Karl-Rudolf ERLEMANN, Regulation of 5-hydroxyeicosanoid dehydrogenase activity in monocytic cells, Biochem. J. (2007) 403, 157–165]
  3. ↑ [http://jpet.aspetjournals.org/content/jpet/329/1/335.full.pdf Karl-Pranav Patel, Selectivity of 5-Hydroxyeicosanoid Dehydrogenase and Its Inhibition by 5-Hydroxy-Long-Chain Fatty Acids, Journal of Pharmacology and Experimental Therapeutics April 2009, 329 (1) 335-341; ]
  4. ↑ W. Bothwell, "A radioimmunoassay for the unstable pulmonary metabolites of prostaglandin E1 and E2: an indirect index of their in vivo disposition and pharmacokinetics" Journal of Pharmacology and Experimental Therapeutics February 1982, 220 (2) 229-235
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