Difference between revisions of "Transformation of 15-Keto-PGE2 to 13,14-Dihydro-15-Keto-PGE2"
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== Parameters == | == Parameters == | ||
− | + | ===K<sub>ms</sub>=== | |
{|class="wikitable" | {|class="wikitable" | ||
− | |+ style="text-align: left;" | | + | |+ style="text-align: left;" | Literature values |
! Value | ! Value | ||
! Units | ! Units | ||
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|} | |} | ||
+ | |||
+ | {| class="wikitable" | ||
+ | |+ style="text-align: left;" | Description of the PTGR-2 Kms distribution | ||
+ | ! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ) | ||
+ | |- | ||
+ | | 1.20E-02 || 4.92E+00 || -3.97E+00 || 6.71E-01 | ||
+ | |} | ||
+ | |||
+ | [[Image:77.jpg|none|thumb|500px|The estimated probability distribution for PTGR-2 Kms. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale. ]] | ||
+ | |||
+ | ===K<sub>mp</sub>=== | ||
+ | {| class="wikitable" | ||
+ | |+ style="text-align: left;" | Description of the PTGR-2 Kmp distribution | ||
+ | ! Mode (mM) !! Location parameter (µ) !! Scale parameter (σ) | ||
+ | |- | ||
+ | | 1.19E-02 || -3.97E+00 || 6.81E-01 | ||
+ | |- | ||
+ | | | ||
+ | |} | ||
+ | |||
+ | [[Image:78.jpg|none|thumb|500px|The estimated probability distribution for PTGR-2 Kmp. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale. ]] | ||
+ | |||
+ | ===k<sub>cat</sub>=== | ||
Note: Turnover values from EC 1.3.1.48 - 13,14-dehydro-15-oxoprostaglandin 13-reductase | Note: Turnover values from EC 1.3.1.48 - 13,14-dehydro-15-oxoprostaglandin 13-reductase | ||
{|class="wikitable" | {|class="wikitable" | ||
− | |+ style="text-align: left;" | | + | |+ style="text-align: left;" | Literature values |
! Value | ! Value | ||
! Units | ! Units | ||
Line 94: | Line 117: | ||
| <ref name="Wu2008"> [http://www.jbc.org/content/282/25/18162.long Identification of a Novel Prostaglandin Reductase Reveals the Involvement of Prostaglandin E2 Catabolism in Regulation of Peroxisome Proliferator-activated Receptor γ Activation, June 22, 2007, The Journal of Biological Chemistry, 282, 18162-18172]</ref> | | <ref name="Wu2008"> [http://www.jbc.org/content/282/25/18162.long Identification of a Novel Prostaglandin Reductase Reveals the Involvement of Prostaglandin E2 Catabolism in Regulation of Peroxisome Proliferator-activated Receptor γ Activation, June 22, 2007, The Journal of Biological Chemistry, 282, 18162-18172]</ref> | ||
|- | |- | ||
+ | |} | ||
+ | {| class="wikitable" | ||
+ | |+ style="text-align: left;" | Description of the PTGR-2 kcat distribution | ||
+ | ! Mode (min-1) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ) | ||
+ | |- | ||
+ | | 1.14E+01 || 9.86E+00 || 3.22E+00 || 8.87E-01 | ||
|} | |} | ||
+ | [[Image:79.jpg|none|thumb|500px|The estimated probability distribution for PTGR-2 kcat. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale. ]] | ||
+ | ===Enzyme concentration === | ||
{|class="wikitable" | {|class="wikitable" | ||
|+ style="text-align: left;" | PTGR-2 Abundance | |+ style="text-align: left;" | PTGR-2 Abundance | ||
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|} | |} | ||
+ | {| class="wikitable" | ||
+ | |+ style="text-align: left;" | Description of the PTGR-2 concentration distribution | ||
+ | ! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ) | ||
+ | |- | ||
+ | | 8.65E+01 || 1.45E+00 || 4.58E+00 || 3.47E-01 | ||
+ | |} | ||
+ | |||
+ | [[Image:168.jpg|none|thumb|500px|The estimated probability distribution for PTGR-2 concentration. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale. ]] | ||
+ | |||
+ | ===K<sub>eq</sub>=== | ||
{|class="wikitable" | {|class="wikitable" | ||
− | |+ style="text-align: left;" | Gibbs Free energy | + | |+ style="text-align: left;" | Literature values |
− | + | ! Gibbs Free energy | |
! Units | ! Units | ||
! Species | ! Species | ||
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|} | |} | ||
+ | {| class="wikitable" | ||
+ | |+ style="text-align: left;" | Description of the PTGR-2 Keq distribution | ||
+ | ! Mode !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ) | ||
+ | |- | ||
+ | | 2.28E-03 || 1.00E+01 || -5.29E+00 || 8.91E-01 | ||
+ | |} | ||
+ | |||
+ | [[Image:80.jpg|none|thumb|500px|The estimated probability distribution for PTGR-2 Keq. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale. ]] | ||
+ | |||
+ | ===Misch=== | ||
{|class="wikitable" | {|class="wikitable" |
Revision as of 14:23, 16 May 2019
PTGR-2 catalyses the reduction of the conjugated α,β-unsaturated double bond of 15-Keto-PGE2. This reaction is NADPH-dependant and generates 13,14-dihydro-15-keto-PGE2.
Contents
Reaction
Chemical equation
Rate equation
Parameters
Kms
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
0.01121 ± 0.00014 | mM | Human | Method: In vitro
Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: 15-Keto-PGE2 "For determining the KM and Vmax values for NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different concentrations of NADPH (0–60 mM)." |
[1] |
0.01587 ± 0.00171 | mM | Human | Method: In vitro
Organism: Human Expression vector: E.coli Enzyme: e PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: NADPH "For determining the KM and Vmax values for NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different concentrations of NADPH (0–60 mM)." |
[1] |
0.0496 ± 0.0058 | mM | Mouse | Method: In vitro
Organism: Mouse Expression vector: Enzyme: 13,14-dehydro-15-oxoprostaglandin 13-reductase pH: 7.4 Temperature: 37 ◦C Substrate: 15-Ketoprostaglandin E2 |
[1] |
Mode (mM) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|
1.20E-02 | 4.92E+00 | -3.97E+00 | 6.71E-01 |
Kmp
Mode (mM) | Location parameter (µ) | Scale parameter (σ) |
---|---|---|
1.19E-02 | -3.97E+00 | 6.81E-01 |
kcat
Note: Turnover values from EC 1.3.1.48 - 13,14-dehydro-15-oxoprostaglandin 13-reductase
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
11.4 ± 0.9 | min-1 | Mouse | Method: In vitro
Organism: Mouse Expression vector: Enzyme: 13,14-dehydro-15-oxoprostaglandin 13-reductase pH: 7.4 Temperature: 37 ◦C Substrate: 15-Ketoprostaglandin E2 |
[1] |
Mode (min-1) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|
1.14E+01 | 9.86E+00 | 3.22E+00 | 8.87E-01 |
Enzyme concentration
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
162 | ppm | Human | Expression Vector: Skin
Enzyme: PTGR2 pH: 7.5 Temperature: 37 °C |
[2] |
80.9 | ppm | Human | Expression Vector: Skin
Enzyme: PTGR2 pH: Unknown Temperature: Unknown |
Unknown |
74.1 | ppm | Human | Expression Vector: Oral Cavity
Enzyme: PTGR2 pH: 7.5 Temperature: 37 °C |
[2] |
Mode (mM) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|
8.65E+01 | 1.45E+00 | 4.58E+00 | 3.47E-01 |
Keq
Gibbs Free energy | Units | Species | Notes | Reference |
---|---|---|---|---|
3.6006165 | kcal/mol | Unspecified | Calculations with a Gaussian98 suite of programs
Enzyme: COX (Unspecific) Substrate: Arachidonate Temperature: 298.15 K Pressure: 1 bar |
[3] |
Mode | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|
2.28E-03 | 1.00E+01 | -5.29E+00 | 8.91E-01 |
Misch
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
9.9 +/-0.2 | minutes | Dog | In vivo
Temperature:37 pH:7 |
[4] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
159.23 ± 0.71 | nmol min-1 mg-1 | Human | Method: In vitro
Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: 15-Keto-PGE2 |
[1] |
66.73 ± 1.36 | nmol min-1 mg-1 | Human | Method: In vitro
Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: NADPH |
[1] |
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2, Structure. 2008 Nov 12;16(11):1714-23. doi: 10.1016/j.str.2008.09.007. Cite error: Invalid
<ref>
tag; name "Wu2008" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Wu2008" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Wu2008" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Wu2008" defined multiple times with different content - ↑ 2.0 2.1 [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587]
- ↑ P. Silva, "A theoretical study of radical-only and combined radical/carbocationic mechanisms of arachidonic acid cyclooxygenation by prostaglandin H synthase" Theor Chem Acc (2003) 110: 345
- ↑ W. Bothwell, "A radioimmunoassay for the unstable pulmonary metabolites of prostaglandin E1 and E2: an indirect index of their in vivo disposition and pharmacokinetics" Journal of Pharmacology and Experimental Therapeutics February 1982, 220 (2) 229-235