Difference between revisions of "Transformation of 15-Keto-PGE2 to 13,14-Dihydro-15-Keto-PGE2"
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− | + | Note: Turnover values from EC 1.3.1.48 - 13,14-dehydro-15-oxoprostaglandin 13-reductase | |
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! Reference | ! Reference | ||
|- | |- | ||
− | | | + | | 162 |
− | | | + | | ppm |
− | | | + | | Human |
− | | | + | |Expression Vector: Skin |
− | | | + | Enzyme: PTGR2 |
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the | ||
+ | human proteome'' Nature, 2014 509, 582–587]</ref> | ||
+ | |- | ||
+ | |80.9 | ||
+ | |ppm | ||
+ | |Human | ||
+ | |Expression Vector: Skin | ||
+ | Enzyme: PTGR2 | ||
+ | pH: Unknown | ||
+ | Temperature: Unknown | ||
+ | |Unknown | ||
+ | |- | ||
+ | |74.1 | ||
+ | |ppm | ||
+ | |Human | ||
+ | |Expression Vector: Skin | ||
+ | Enzyme: PTGR2 | ||
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the | ||
+ | human proteome'' Nature, 2014 509, 582–587]</ref> | ||
+ | |- | ||
|} | |} | ||
Revision as of 15:30, 15 March 2017
Reaction
Chemical equation
Rate equation
Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
0.01121 ± 0.00014 | mM | Human | Method: In vitro
Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: 15-Keto-PGE2 "For determining the KM and Vmax values for NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different concentrations of NADPH (0–60 mM)." |
[1] |
0.01587 ± 0.00171 | mM | Human | Method: In vitro
Organism: Human Expression vector: E.coli Enzyme: e PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: NADPH "For determining the KM and Vmax values for NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different concentrations of NADPH (0–60 mM)." |
[1] |
0.0496 ± 0.0058 | mM | Mouse | Method: In vitro
Organism: Mouse Expression vector: Enzyme: 13,14-dehydro-15-oxoprostaglandin 13-reductase pH: 7.4 Temperature: 37 ◦C Substrate: 15-Ketoprostaglandin E2 |
[1] |
Note: Turnover values from EC 1.3.1.48 - 13,14-dehydro-15-oxoprostaglandin 13-reductase
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
11.4 ± 0.9 | min-1 | Mouse | Method: In vitro
Organism: Mouse Expression vector: Enzyme: 13,14-dehydro-15-oxoprostaglandin 13-reductase pH: 7.4 Temperature: 37 ◦C Substrate: 15-Ketoprostaglandin E2 |
[1] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
162 | ppm | Human | Expression Vector: Skin
Enzyme: PTGR2 pH: 7.5 Temperature: 37 °C |
[2] |
80.9 | ppm | Human | Expression Vector: Skin
Enzyme: PTGR2 pH: Unknown Temperature: Unknown |
Unknown |
74.1 | ppm | Human | Expression Vector: Skin
Enzyme: PTGR2 pH: 7.5 Temperature: 37 °C |
[2] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
X | Y | Z | A | B |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
9.9 +/-0.2 | minutes | Dog | In vivo
Temperature:37 pH:7 |
[3] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
159.23 ± 0.71 | nmol min-1 mg-1 | Human | Method: In vitro
Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: 15-Keto-PGE2 |
[1] |
66.73 ± 1.36 | nmol min-1 mg-1 | Human | Method: In vitro
Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: NADPH |
[1] |
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2, Structure. 2008 Nov 12;16(11):1714-23. doi: 10.1016/j.str.2008.09.007. Cite error: Invalid
<ref>
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tag; name "Wu2008" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Wu2008" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Wu2008" defined multiple times with different content - ↑ 2.0 2.1 [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587]
- ↑ W. Bothwell, "A radioimmunoassay for the unstable pulmonary metabolites of prostaglandin E1 and E2: an indirect index of their in vivo disposition and pharmacokinetics" Journal of Pharmacology and Experimental Therapeutics February 1982, 220 (2) 229-235