Difference between revisions of "Transformation of 15-Keto-PGE2 to 13,14-Dihydro-15-Keto-PGE2"

From ISMOC
Jump to: navigation, search
(→‎Chemical equation)
(→‎Parameters)
Line 57: Line 57:
 
| <ref name="Wu2008"> [https://www.ncbi.nlm.nih.gov/pubmed/19000823 Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2, Structure. 2008 Nov 12;16(11):1714-23. doi: 10.1016/j.str.2008.09.007.]</ref>  
 
| <ref name="Wu2008"> [https://www.ncbi.nlm.nih.gov/pubmed/19000823 Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2, Structure. 2008 Nov 12;16(11):1714-23. doi: 10.1016/j.str.2008.09.007.]</ref>  
 
|-
 
|-
−
 
+
|-
 +
|0.0496 ± 0.0058
 +
|mM
 +
| Mouse
 +
|Method: In vitro
 +
Organism: Mouse
 +
Expression vector:
 +
Enzyme: 13,14-dehydro-15-oxoprostaglandin 13-reductase
 +
pH: 7.4
 +
Temperature:  37 ◦C
 +
Substrate: 15-Ketoprostaglandin E2
 +
| <ref name="Wu2008"> [http://www.jbc.org/content/282/25/18162.long Identification of a Novel Prostaglandin Reductase Reveals the Involvement of Prostaglandin E2 Catabolism in Regulation of Peroxisome Proliferator-activated Receptor γ Activation, June 22, 2007, The Journal of Biological Chemistry, 282, 18162-18172]</ref>
 +
|-
 
|}
 
|}
  
−
 
+
Values from EC 1.3.1.48 - 13,14-dehydro-15-oxoprostaglandin 13-reductase
  
 
{|class="wikitable"  
 
{|class="wikitable"  
Line 70: Line 82:
 
! Reference
 
! Reference
 
|-
 
|-
−
| X
+
| 11.4 ± 0.9
−
| Y
+
| min-1
−
| Z
+
| Mouse
−
| A
+
|Method: In vitro
−
| B
+
Organism: Mouse
 +
Expression vector:
 +
Enzyme: 13,14-dehydro-15-oxoprostaglandin 13-reductase
 +
pH: 7.4
 +
Temperature:  37 ◦C
 +
Substrate: 15-Ketoprostaglandin E2
 +
| <ref name="Wu2008"> [http://www.jbc.org/content/282/25/18162.long Identification of a Novel Prostaglandin Reductase Reveals the Involvement of Prostaglandin E2 Catabolism in Regulation of Peroxisome Proliferator-activated Receptor γ Activation, June 22, 2007, The Journal of Biological Chemistry, 282, 18162-18172]</ref>
 +
|-
 +
 
 
|}
 
|}
 +
 +
 
{|class="wikitable"  
 
{|class="wikitable"  
 
|+  style="text-align: left;" | 5-HEDH Abundance  
 
|+  style="text-align: left;" | 5-HEDH Abundance  

Revision as of 10:37, 15 March 2017

Return to overview


Reaction

Chemical equation

15-Keto-PGE2 + NAD(P)+ \rightleftharpoons 13,14-Dihydro-15-Keto-PGE2 + NAD(P)H

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
0.01121 ± 0.00014 mM Human Method: In vitro

Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: 15-Keto-PGE2

"For determining the KM and Vmax values for NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different concentrations of NADPH (0–60 mM)."

[1]
0.01587 ± 0.00171 mM Human Method: In vitro

Organism: Human Expression vector: E.coli Enzyme: e PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: NADPH

"For determining the KM and Vmax values for NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different concentrations of NADPH (0–60 mM)."

[1]
0.0496 ± 0.0058 mM Mouse Method: In vitro

Organism: Mouse Expression vector: Enzyme: 13,14-dehydro-15-oxoprostaglandin 13-reductase pH: 7.4 Temperature: 37 ◦C Substrate: 15-Ketoprostaglandin E2

[1]

Values from EC 1.3.1.48 - 13,14-dehydro-15-oxoprostaglandin 13-reductase

Enzyme Turnover Number
Value Units Species Notes Reference
11.4 ± 0.9 min-1 Mouse Method: In vitro

Organism: Mouse Expression vector: Enzyme: 13,14-dehydro-15-oxoprostaglandin 13-reductase pH: 7.4 Temperature: 37 ◦C Substrate: 15-Ketoprostaglandin E2

[1]


5-HEDH Abundance
Value Units Species Notes Reference
X Y Z A B
Gibbs Free energy
Value Units Species Notes Reference
X Y Z A B


Half Life
Value Units Species Notes Reference
9.9 +/-0.2 minutes Dog In vivo

Temperature:37 pH:7

[2]
Vmax
Value Units Species Notes Reference
159.23 ± 0.71 nmol min-1 mg-1 Human Method: In vitro

Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: 15-Keto-PGE2

[1]
66.73 ± 1.36 nmol min-1 mg-1 Human Method: In vitro

Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: NADPH

[1]

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2, Structure. 2008 Nov 12;16(11):1714-23. doi: 10.1016/j.str.2008.09.007. Cite error: Invalid <ref> tag; name "Wu2008" defined multiple times with different content Cite error: Invalid <ref> tag; name "Wu2008" defined multiple times with different content Cite error: Invalid <ref> tag; name "Wu2008" defined multiple times with different content Cite error: Invalid <ref> tag; name "Wu2008" defined multiple times with different content
  2. ↑ W. Bothwell, "A radioimmunoassay for the unstable pulmonary metabolites of prostaglandin E1 and E2: an indirect index of their in vivo disposition and pharmacokinetics" Journal of Pharmacology and Experimental Therapeutics February 1982, 220 (2) 229-235
Retrieved from ‘http://www.systemsbiology.ls.manchester.ac.uk/wiki/index.php?title=Transformation_of_15-Keto-PGE2_to_13,14-Dihydro-15-Keto-PGE2&oldid=5162’