Transformation of 15-Keto-PGE2 to 13,14-Dihydro-15-Keto-PGE2

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Chemical equation

$15-Keto-PGE2 + NAD(P)+ \rightleftharpoons 13,14-Dihydro-15-Keto-PGE2 + NAD(P)H$

Parameters

Michaelis-Menten Constants

Value	Units	Species	Notes	Reference
0.01121 ± 0.00014	mM	Human	Method: In vitro Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: 15-Keto-PGE2 "For determining the KM and Vmax values for NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different concentrations of NADPH (0–60 mM)."
0.01587 ± 0.00171	mM	Human	Method: In vitro Organism: Human Expression vector: E.coli Enzyme: e PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: NADPH "For determining the KM and Vmax values for NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different concentrations of NADPH (0–60 mM)."

Enzyme Turnover Number

Value	Units	Species	Notes	Reference
X	Y	Z	A	B

5-HEDH Abundance

Value	Units	Species	Notes	Reference
X	Y	Z	A	B

Gibbs Free energy

Value	Units	Species	Notes	Reference
X	Y	Z	A	B

Half Life

Value	Units	Species	Notes	Reference
9.9 +/-0.2	minutes	Dog	In vivo Temperature:37 pH:7

Vmax

Value	Units	Species	Notes	Reference
159.23 ± 0.71	nmol min-1 mg-1	Human	Method: In vitro Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: 15-Keto-PGE2
66.73 ± 1.36	nmol min-1 mg-1	Human	Method: In vitro Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: NADPH