Difference between revisions of "Transformation of 15-Keto-PGE2 to 13,14-Dihydro-15-Keto-PGE2"

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pH:7
 
pH:7
 
| <ref name="Bothwell1982”>[http://jpet.aspetjournals.org/content/220/2/229.long W. Bothwell, "A radioimmunoassay for the unstable pulmonary metabolites of prostaglandin E1 and E2: an indirect index of their in vivo disposition and pharmacokinetics" Journal of Pharmacology and Experimental Therapeutics February 1982, 220 (2) 229-235]</ref>
 
| <ref name="Bothwell1982”>[http://jpet.aspetjournals.org/content/220/2/229.long W. Bothwell, "A radioimmunoassay for the unstable pulmonary metabolites of prostaglandin E1 and E2: an indirect index of their in vivo disposition and pharmacokinetics" Journal of Pharmacology and Experimental Therapeutics February 1982, 220 (2) 229-235]</ref>
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{|class="wikitable"
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|+  style="text-align: left;" | Vmax
 +
! Value
 +
! Units
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! Species
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! Notes
 +
! Reference
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|-
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| 159.23 ± 0.71
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| nmol min-1 mg-1
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| Human
 +
|Method: In vitro
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Organism: Human
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Expression vector: E.coli
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Enzyme: PTGR2
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pH: 7.5
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Temperature:  37 ◦C
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Substrate: 15-Keto-PGE2
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| <ref name="Wu2008"> [https://www.ncbi.nlm.nih.gov/pubmed/19000823 Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2, Structure. 2008 Nov 12;16(11):1714-23. doi: 10.1016/j.str.2008.09.007.]</ref>
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|-
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| 66.73 ± 1.36
 +
| nmol min-1 mg-1
 +
| Human
 +
|Method: In vitro
 +
Organism: Human
 +
Expression vector: E.coli
 +
Enzyme: PTGR2
 +
pH: 7.5
 +
Temperature:  37 ◦C
 +
Substrate: NADPH
 +
| <ref name="Wu2008"> [https://www.ncbi.nlm.nih.gov/pubmed/19000823 Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2, Structure. 2008 Nov 12;16(11):1714-23. doi: 10.1016/j.str.2008.09.007.]</ref>
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|-
 
|}
 
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Revision as of 10:43, 13 March 2017

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Reaction

Chemical equation

Failed to parse (Cannot store math image on filesystem.): 15-Keto-PGE2 \rightleftharpoons 13,14-Dihydro-15-Keto-PGE2

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
0.01121 ± 0.00014 mM Human Method: In vitro

Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: 15-Keto-PGE2

"For determining the KM and Vmax values for NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different concentrations of NADPH (0–60 mM)."

[1]
0.01587 ± 0.00171 mM Human Method: In vitro

Organism: Human Expression vector: E.coli Enzyme: e PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: NADPH

"For determining the KM and Vmax values for NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different concentrations of NADPH (0–60 mM)."

[1]


Enzyme Turnover Number
Value Units Species Notes Reference
X Y Z A B
5-HEDH Abundance
Value Units Species Notes Reference
X Y Z A B
Gibbs Free energy
Value Units Species Notes Reference
X Y Z A B


Half Life
Value Units Species Notes Reference
9.9 +/-0.2 minutes Dog In vivo

Temperature:37 pH:7

[2]
Vmax
Value Units Species Notes Reference
159.23 ± 0.71 nmol min-1 mg-1 Human Method: In vitro

Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: 15-Keto-PGE2

[1]
66.73 ± 1.36 nmol min-1 mg-1 Human Method: In vitro

Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: NADPH

[1]
  1. ↑ 1.0 1.1 1.2 1.3 Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2, Structure. 2008 Nov 12;16(11):1714-23. doi: 10.1016/j.str.2008.09.007.
  2. ↑ W. Bothwell, "A radioimmunoassay for the unstable pulmonary metabolites of prostaglandin E1 and E2: an indirect index of their in vivo disposition and pharmacokinetics" Journal of Pharmacology and Experimental Therapeutics February 1982, 220 (2) 229-235
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